The Neuroprotective Effect of Overexpression of Calbindin-D28k in an Animal Model of Parkinson’s Disease
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Overexpression of calbindin-D28k (CaBP-28 k) induces neurite outgrowth in dopaminergic neuronal cells and could provide some protection to dopaminergic neurons against the pathological process in Parkinson’s disease. Transgenic mice CaBP-28 k overexpression and the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced mouse models were generated, and the effect of midbrain dopamine neurons in ethology was also assessed. Tyrosine hydroxylase (TH)-immunoreactive neurons were counted, and the concentration of total protein and dopamine (DA) of striatum corpora was measured in four animal models. Results showed that the positive TH cells, content of DA, and ability of ethology in MPTP-induced transgenic mice were significantly higher than that in MPTP-induced wild-type mice. The findings demonstrate that overexpression of CaBP-28 k could provide protection for DA neurons from neurodegeneration. It would provide a potential strategy in the treatment of Parkinson’s diseases.
KeywordsCalbindin-D28k Dopamine Parkinson’s disease Transgenic mice
The research was financially supported by the National Natural Science Foundation of China (No. 31172171), the Natural Science Foundation of Jiangsu Province (No. BK2010180); the President Foundation of Xuzhou Medical College (No. 2010KJZ10), and Social Development Project of Xuzhou (No. XM09B116).
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