Synthesis and characterization of SPIO-loaded PEG-b-PS micelles as contrast agent for long-term nanoparticle-based MRI phantom

  • Man Theerasilp
  • Witaya Sungkarat
  • Norased Nasongkla


Nanoparticle-based magnetic resonance imaging (MRI) phantom was developed from the suspension of magnetic nanoparticles composing methoxy poly(ethylene glycol)-block-poly(styrene) (PEG-b-PS) micelles and superparamagnetic iron oxide (SPIO) nanoparticles in the core of micelles. The size of SPIO-loaded micelles was determined by dynamic light scattering (DLS) and transmission electron microscopy. Larger-size micelles were found when SPIO loading was increased. The effect of the hydrophobic section of block copolymer on the size of micelles was studied by DLS. Transverse relaxivity was evaluated on both 1.5 and 3 T clinical MRI scanner. Higher SPIO-loading provided higher relaxivity, where the relaxivity of 10% SPIO-loaded PEG(5 kDa)-b-PS(5 kDa) were 144.0 and \(174.0~\hbox {m M}^{-1}~\hbox {s}^{-1}\) for 1.5 and 3 T MRI, respectively. This formulation showed stability over a 10-week period, and the standard deviations of the relaxivities were 3.0 and 8.0% for 1.5 and 3 T MRI, respectively. Thus, SPIO-loaded PEG-b-PS micelles have a potential to be applied as a contrast agent for nanoparticle-based MRI phantom.


Iron oxide nanoparticle polymeric micelles stability relaxivity MRI phantom 



This research was supported by Mahidol University, Thailand.


  1. 1.
    Alústiza Echeverría J M, Castiella A and Emparanza J I 2012 Insights Imaging 3 173CrossRefGoogle Scholar
  2. 2.
    Anderson L J, Holden S, Davis B, Prescott E, Charrier C C, Bunce N H et al 2001 Eur. Heart J. 22 2171CrossRefGoogle Scholar
  3. 3.
    Yoshimura K, Kato H, Kuroda M, Yoshida A, Hanamoto K, Tanaka A et al 2003 Magn. Reson. Med. 50 1011CrossRefGoogle Scholar
  4. 4.
    Vassiliou V S, Heng E L, Gatehouse P D, Donovan J, Raphael C E, Giri S et al 2016 J. Cardiovasc. Magn. Reson. 18 1CrossRefGoogle Scholar
  5. 5.
    Alústiza J M, Emparanza J I, Castiella A, Casado A, Garrido A, Aldazábal P et al 2015 Biomed Res. Int. 2015 Article no. 294024Google Scholar
  6. 6.
    Ernst O, Rose C, Sergent G and L’Herminé C 1999 Am. J. Roentgenol. 172 1141CrossRefGoogle Scholar
  7. 7.
    Sreeram K J, Nidhin M, Indumathy R and Nair B U 2008 Bull. Mater. Sci. 31 93CrossRefGoogle Scholar
  8. 8.
    Duguet E, Vasseur S, Mornet S, Goglio G, Demourgues A, Portier J et al 2006 Bull. Mater. Sci. 29 581CrossRefGoogle Scholar
  9. 9.
    Puntawee S, Theerasilp M, Reabroi S, Saeeng R, Piyachaturawat P, Chairoungdua A et al 2016 Pharm. Dev. Technol. 21 437Google Scholar
  10. 10.
    Nasongkla N, Shuai X, Ai H, Weinberg B D, Pink J, Boothman D A et al 2004 Angewandte Chemie—International Edition 43 6323CrossRefGoogle Scholar
  11. 11.
    Blanco E, Kessinger C W, Sumer B D and Gao J 2009 Exp. Biol. Med. 234 123CrossRefGoogle Scholar
  12. 12.
    Ai H, Flask C, Weinberg B, Shuai X, Pagel M D, Farrell D et al 2005 Adv. Mater. 17 1949CrossRefGoogle Scholar
  13. 13.
    Huh K M, Lee S C, Cho Y W, Lee J, Jeong J H and Park K 2005 J. Control. Release 101 59CrossRefGoogle Scholar
  14. 14.
    Theerasilp M, Chalermpanapun P, Ponlamuangdee K, Sukvanitvichai D and Nasongkla N 2017 RSC Adv. 7 11158CrossRefGoogle Scholar
  15. 15.
    Sun S, Zeng H, Robinson D B, Raoux S, Rice P M, Wang S X et al 2004 J. Am. Chem. Soc. 126 273CrossRefGoogle Scholar
  16. 16.
    Jankova K, Chen X, Kops J and Batsberg W 1998 Macromolecules 31 538CrossRefGoogle Scholar
  17. 17.
    Crecelius A C, Becer C R, Knop K and Schubert U S 2010 J. Polym. Sci. Pol. Chem. 48 4375CrossRefGoogle Scholar
  18. 18.
    Kharisov B I, Dias H V R, Kharissova O V, Vázquez A, Peña Y and Gómez I 2014 RSC Adv. 4 45354CrossRefGoogle Scholar
  19. 19.
    Theerasilp M and Nasongkla N 2013 J. Microencapsul. 30 390CrossRefGoogle Scholar
  20. 20.
    Rutnakornpituk M, Meerod S, Boontha B and Wichai U 2009 Polymer 50 3508CrossRefGoogle Scholar
  21. 21.
    Ditsch A, Laibinis P E, Wang D I C and Hatton T A 2005 Langmuir 21 6006CrossRefGoogle Scholar
  22. 22.
    Golas P L, Louie S, Lowry G V, Matyjaszewski K and Tilton R D 2010 Langmuir 26 16890CrossRefGoogle Scholar
  23. 23.
    Lim J, Yeap S P, Che H X and Low S C 2013 Nanoscale Res. Lett. 8 1CrossRefGoogle Scholar
  24. 24.
    Aqil A, Vasseur S, Duguet E, Passirani C, Benoît J P, Roch A et al 2008 Euro. Polym. J. 44 3191CrossRefGoogle Scholar
  25. 25.
    Shuai X, Merdan T, Schaper A K, Xi F and Kissel T 2004 Bioconjugate Chem. 15 441CrossRefGoogle Scholar
  26. 26.
    Astafieva I, Khougaz K and Eisenberg A 1995 Macromolecules 28 7127CrossRefGoogle Scholar
  27. 27.
    Cheng D, Hong G, Wang W, Yuan R, Ai H, Shen J et al 2011 J. Mater. Chem. 21 4796CrossRefGoogle Scholar
  28. 28.
    Guardia P, Labarta A and Batlle X 2011 J. Phys. Chem. C 115 390CrossRefGoogle Scholar
  29. 29.
    Nasongkla N, Bey E, Ren J, Ai H, Khemtong C, Guthi J S et al 2006 Nano Lett. 6 2427CrossRefGoogle Scholar
  30. 30.
    Chen H, Yeh J, Wang L, Khurshid H, Peng N, Wang A Y et al 2010 Nano Res. 3 852CrossRefGoogle Scholar
  31. 31.
    Pöselt E, Kloust H, Tromsdorf U, Janschel M, Hahn C, Maßlo C et al 2012 ACS Nano 6 1619CrossRefGoogle Scholar
  32. 32.
    Duan H, Kuang M, Wang X, Wang Y A, Mao H and Nie S 2008 J. Phys. Chem. C 112 8127CrossRefGoogle Scholar
  33. 33.
    Zhang C, Xie X, Liang S, Li M, Liu Y and Gu H 2012 Magn. Reson. Med. 8 996Google Scholar
  34. 34.
    Arbab A S, Wilson L B, Ashari P, Jordan E K, Lewis B K and Frank J A 2005 NMR Biomed. 18 383CrossRefGoogle Scholar
  35. 35.
    Shapiro M G, Atanasijevic T, Faas H, Westmeyer G G and Jasanoff A 2006 Magn. Reson. Imaging 24 449CrossRefGoogle Scholar

Copyright information

© Indian Academy of Sciences 2018

Authors and Affiliations

  • Man Theerasilp
    • 1
    • 2
  • Witaya Sungkarat
    • 3
  • Norased Nasongkla
    • 1
    • 2
  1. 1.Department of Biomedical Engineering, Faculty of EngineeringMahidol UniversityPhutthamonthonThailand
  2. 2.Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of ScienceMahidol UniversityBangkokThailand
  3. 3.Department of Radiology, Faculty of Medicine, Ramathibodi HospitalMahidol UniversityBangkokThailand

Personalised recommendations