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Cellular Lysosomes’ Activity for Melanin Reduction on Artificial Skin Tissue

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Abstract

All eukaryotes have lysosomes which contain hydrolytic enzymes such as protease to degrade waste materials and cellular fragments. As a cellular organelle, lysosomes function as the digestive system of the cell, serving both to degrade material taken up from outside the cell and to digest obsolete components of the cell itself. Conversely, melanin has photochemical functions to protect tissue from the harmful effects of ultraviolet rays. However, too much of melanin leads to problems such as hyperpigmentation, requiring materials to maintain and control the amount of melanin. In this study, we found evidence of correlation between lysosome and melanin in a new eco-friendly material, MelanoDerm, a reconstituted 3D human skin model containing normal melanocytes and keratinocytes. Melanin content assay and cell viability were measured, using 2% kojic acid as positive control, while MelanoDerm was exposed to various concentrations of lysosome. Our results indicate that lysosome may be a useful cosmetic agent for the treatment of hyperpigmentation.

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Acknowledgements

This research was supported by the Ministry of Trade, Industry & Energy (MOTIE) and Korea Institute for Advancement of Technology (KIAT) through the Encouragement Program (P0006145) for The Industries of Economic Cooperation Region.

Author information

DJP, GJ, SHB, YHK, and JM designed the experiments and analyzed the results. SYK analyzed the experimental data. JHW conducted the artificial tissue analysis. All authors contributed to writing of the manuscript.

Correspondence to Yang-Hoon Kim or Jiho Min.

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The authors declared that they have no conflict of interest.

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Park, D.J., Jeon, G., Bang, S.H. et al. Cellular Lysosomes’ Activity for Melanin Reduction on Artificial Skin Tissue. Mol Biotechnol 62, 185–191 (2020). https://doi.org/10.1007/s12033-019-00235-w

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Keywords

  • Melanin reduction
  • Lysosome
  • Lysosomal enzyme
  • MelanoDerm
  • Artificial skin tissue