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Molecular Biotechnology

, Volume 61, Issue 2, pp 111–121 | Cite as

Generation and Characterization of Fibroblast-Specific Basigin Knockout Mice

  • Xiao-Dong Wu
  • Meng-Yao Zhang
  • Ya-Tong Chen
  • Hui Yao
  • Qing Zhang
  • Wen-Jing Wang
  • Da-Fu Fu
  • Ren-Ji Wei
  • Jia-Yu Zhang
  • Yin Li
  • Dan Dang
  • Hui-Jie Bian
  • Jing XuEmail author
  • Zhi-Nan ChenEmail author
Original Paper
  • 53 Downloads

Abstract

Basigin is a well-known extracellular stimulator of fibroblasts and may confer resistance to apoptosis of fibroblasts in vitro under some pathological status, but its exact function in fibroblasts and the underlying mechanism remain poorly understood. The systematic Basigin gene knockout leads to the perinatal lethality of mice, which limits the delineation of its function in vivo. In this study, we generated a fibroblast-specific Basigin knock-out mouse model and demonstrated the successful deletion of Basigin in fibroblasts. The fibroblast-specific deletion of Basigin did not influence the growth, fertility and the general condition of the mice. No obvious differences were found in the size, morphology, and histological structure of the major organs, including heart, liver, spleen, lung and kidney, between the knockout mice and the control mice. The deletion of Basigin in fibroblasts did not induce apoptosis in the tissues of the major organs. These results provide the first evidence that the fibroblast-specific Basigin knock-out mice could be a useful tool for exploring the function of Basigin in fibroblasts in vivo.

Keywords

Basigin Fibroblast Conditional gene knockout mice Apoptosis Cre-loxp 

Notes

Funding

This work was supported by the National Natural Science Foundation of China (31371376, 81572802, 81372595), the National Basic Research Program of China (2015CB553700) and the State Key Laboratory of Cancer Biology Project (CBSKL2017Z06).

Compliance with Ethical Standards

Conflict of Interest

The authors declare no conflicts of interest in this work.

Ethical Approval

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. All procedures performed in studies involving animals were in accordance with the ethical standards of the institution or practice at which the studies were conducted. This article does not contain any studies with human participants performed by any of the authors.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Cell Biology, National Translational Science Center for Molecular Medicine, State Key Laboratory of Cancer BiologyFourth Military Medical UniversityXi’anChina
  2. 2.Center of Anesthesiology & OperationChinese PLA General HospitalBeijingChina
  3. 3.Beijing Institute of BiotechnologyBeijingChina
  4. 4.Department of Radiation OncologyThe First Peoples’ Hospital of Changzhou, The Third Affiliated Hospital of Soochow UniversityChangzhouChina
  5. 5.Institute of Liver SurgeryGeneral Hospital of Chinese People’s Armed Police ForcesBeijingChina

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