A Graft Mimic Strategy for Verticillium Resistance in Tomato
Grafting vegetables for disease resistance has increased greatly in popularity over the past 10 years. Verticillium wilt of tomato is commonly controlled through grafting of commercial varieties on resistant rootstocks expressing the Ve1 R-gene. To mimic the grafted plant, proteomic analyses in tomato were used to identify a suitable root-specific promoter (TMVi), which was used to express the Ve1-allele in susceptible Craigella (Cs) tomato plants. The results indicate that when infected with Verticillim dahliae, race 1, the transformed plants are comparable to resistant cultivars (Cr) or grafted plants.
KeywordsPlant grafting Proteome Tissue-specific expression Tomato Verticillium wilt Ve R-locus Ve1-gene
We thank Drs. X. Luo and K.V. Soman (UTMB Proteomics Center) for the peptide mass spectrometry and help with the proteomic analyses. This study was supported by NSERC Canada (R.N.N. and E.J.R.) and NIH, NHLBI (A.K.).
RNN and JR conceived the experiments and prepared the manuscript, MM conducted the experiments. RNN conducted the data analyses and AK was responsible for the peptide mass spectrometry.
- 1.Rivard, C. L., & Louws, F. J. (2011) Tomato grafting: A new tool for disease resistance and increased productivity. North Carolina: SARE Ag Innovation Bulletin Series.Google Scholar
- 3.Schaible, L., Cannon, O. S., & Waddoups, V. (1951). Inheritance of resistance to Verticillium wilt in a tomato cross. Phytopathology, 41, 986–990.Google Scholar
- 16.Pichersky, E., Bernatzky, R., Tanksley, S. D., Cashmore, A. R. (1986) Evidence for selection as a mechanism in the concerted evolution of Lycopersicon esculentum (tomato) genes encoding the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase. Proceedings of the National Academy of Sciences of the United States of America, 83, 3880–3884.CrossRefGoogle Scholar
- 17.Sugita, M., Manzara, T., Pichersky, E., Cashmore, A., & Gruissem, W. (1987). Genomic organization, sequence analysis and expression of all five genes encoding the small subunit of ribulose-l,5-bisphosphate carboxylase/oxygenase from tomato. Molecular and General Genetics, 209, 247–256.CrossRefGoogle Scholar
- 20.Shin, R., An, J. M., Park, C. J., Kim, Y. J., Joo, S., Kim, W. T., & Paek, K.H. (2004). Capsicum annuum tobacco mosaic virus-induced clone 1 expression perturbation alters the plant’s response to ethylene and interferes with the redox homeostasis. The Plant Journal, 37, 186–198.CrossRefGoogle Scholar