Molecular Biotechnology

, Volume 60, Issue 8, pp 533–538 | Cite as

Pellino-1 Protects Periodontal Ligament Stem Cells Against H2O2-Induced Apoptosis via Activation of NF-κB Signaling

  • Jiangang Tian
  • Liufang Gu
  • Andrew Adams
  • Xueliang WangEmail author
  • Ruizhe HuangEmail author
Original Paper


To determine the protective effects of Pellino-1 against H2O2-induced apoptosis in periodontal ligament stem cells (PDLSC). We demonstrated that H2O2 decreases PDLSC viability by 40 and 50% with the concentrations of 400 and 500 μM, respectively, with an observed downregulation of Pellino-1 mRNA and protein; we further concluded that overexpression of Pellino-1 significantly lowers 8-hydroxy-2′-deoxyguanosine levels by 10% and upregulates superoxide dismutase 1, glutathione peroxidase levels, and catalase mRNA levels by 200, 40, and 250%, respectively. More importantly, we found that overexpression of Pellino-1 inhibited H2O2-induced cellular apoptosis through the activation of the NF-κB signaling pathway. Pellino-1 may be critically important for cell survival in the presence of oxidative elements; activation of the NF-κB signaling cascade was required for the overexpression of Pellino-1 to protect the cells from H2O2-induced apoptosis.


Biochemistry DNA damage/repair Oxidative stress 



B-cell lymphoma 6


Dichlorofluorescin diacetate


Glutathione peroxidase


Hydrogen peroxide


3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide


Nuclear factor kappa-light-chain-enhancer of activated B cells




Periodontal ligament


Periodontal ligament stem cells


Reactive oxygen species


Superoxide dismutases


Toll-like receptors


Interleukin-1 receptor


Mitogen-activated protein kinases

Supplementary material

12033_2018_67_MOESM1_ESM.docx (19 kb)
Supplementary material 1 (DOCX 19 kb)


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, College of StomatologyXi’an Jiaotong UniversityXi’anChina
  2. 2.Department of HematologyThe Second Affiliated Hospital of Xi’an Jiaotong UniversityXi’anChina
  3. 3.Institute of Veterinary PhysiologyUniversity of ZurichZurichSwitzerland
  4. 4.Department of Epidemiology and Biostatistics, School of Public HealthXi’an Jiaotong University Health Science CenterXi’anChina

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