Molecular Biotechnology

, Volume 57, Issue 6, pp 489–498

A Low-Affinity K+ Transporter AlHKT2;1 from Recretohalophyte Aeluropus lagopoides Confers Salt Tolerance in Yeast

  • Payal Sanadhya
  • Parinita Agarwal
  • Jackson Khedia
  • Pradeep K. Agarwal
Research

Abstract

The high-affinity potassium transporters (HKT) are highly important for stress tolerance in plants as they uniquely maintain K+/Na+ ratio for their survival and growth. In this study a novel HKT gene AlHKT2;1 was isolated and characterized from salt secreting halophyte, Aeluropus lagopoides. The AlHKT2;1 cDNA comprised of an open reading frame of 1,581 bp, encoding a protein of 526 amino acid residues. It belongs to class II HKTs and showed high homology with other HKT genes. Functional characterization of AlHKT2;1 in both K+ uptake‐deficient (WΔ6) and Na+-sensitive yeast mutants (G19) showed the characteristic feature of low-affinity K+ transporter supporting the growth at >1 mM KCl concentration. The transformed yeast cells showed high sensitivity to NaCl; however, the addition of KCl along with NaCl support the growth of AlHKT2;1 expressing mutant. Ion content analysis of yeast cells with AlHKT2;1 grown in high NaCl medium supplemented with KCl revealed that salt tolerance was correlated with accumulation of K+ during salt stress. These results suggest that AlHKT2;1 plays an important role in the K+ uptake during salt stress and in maintaining a high K+/Na+ ratio in the cytosol.

Keywords

Aeluropus lagopoides HKT transporter Salt stress AlHKT2;1 S. cerevisiae 

Supplementary material

12033_2015_9842_MOESM1_ESM.docx (12 kb)
Supplementary material 1 (DOCX 12 kb)
12033_2015_9842_MOESM2_ESM.pptx (807 kb)
Supplementary material 2 (PPTX 806 kb)

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Payal Sanadhya
    • 1
    • 2
  • Parinita Agarwal
    • 1
  • Jackson Khedia
    • 1
    • 2
  • Pradeep K. Agarwal
    • 1
    • 2
  1. 1.Discipline of Wasteland Research, CSIR-Central Salt and Marine Chemicals Research Institute (CSIR-CSMCRI)Council of Scientific and Industrial Research (CSIR)BhavnagarIndia
  2. 2.Academy of Scientific and Innovative Research, CSIR-Central Salt and Marine Chemicals Research Institute (CSIR-CSMCRI)Council of Scientific and Industrial Research (CSIR)BhavnagarIndia

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