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Molecular Biotechnology

, Volume 57, Issue 1, pp 12–26 | Cite as

Basic Leucine Zipper Family in Barley: Genome-Wide Characterization of Members and Expression Analysis

  • Ehsan Pourabed
  • Farzan Ghane Golmohamadi
  • Peyman Soleymani Monfared
  • Seyed Morteza Razavi
  • Zahra-Sadat ShobbarEmail author
Research

Abstract

The basic leucine zipper (bZIP) family is one of the largest and most diverse transcription factors in eukaryotes participating in many essential plant processes. We identified 141 bZIP proteins encoded by 89 genes from the Hordeum vulgare genome. HvbZIPs were classified into 11 groups based on their DNA-binding motif. Amino acid sequence alignment of the HvbZIPs basic-hinge regions revealed some highly conserved residues within each group. The leucine zipper heptads were analyzed predicting their dimerization properties. 34 conserved motifs were identified outside the bZIP domain. Phylogenetic analysis indicated that major diversification within the bZIP family predated the monocot/dicot divergence, although intra-species duplication and parallel evolution seems to be occurred afterward. Localization of HvbZIPs on the barley chromosomes revealed that different groups have been distributed on seven chromosomes of barley. Six types of intron pattern were detected within the basic-hinge regions. Most of the detected cis-elements in the promoter and UTR sequences were involved in seed development or abiotic stress response. Microarray data analysis revealed differential expression pattern of HvbZIPs in response to ABA treatment, drought, and cold stresses and during barley grain development and germination. This information would be helpful for functional characterization of bZIP transcription factors in barley.

Keywords

Hordeum vulgare Basic leucine zipper Phylogenetic analysis Affymetrix barley1 gene chip 

Abbreviations

aa

Amino acids

ABA

Abscisic acid

ABF

ABRE-binding factors

ABRE

ABA-responsive elements

AREB

ABRE-binding proteins

bZIP

Basic leucine zipper

CV

Coefficient of variation

DRE

Dehydration-responsive element

IBSC

International barley sequencing consortium

Leu

Leucine

MLS

Mean of log-signal values

nt

Nucleotide

ORF

Open reading frame

P0

Phase 0

P2

Phase 2

TF

Transcription factor

uATG

Upstream ATG

uORF

Upstream ORF

UTR

Untranslated region

Notes

Acknowledgments

This work was supported by Agricultural Biotechnology Research Institute of Iran (ABRII). The authors are grateful to Faezeh Tamimi for her technical assistance.

Supplementary material

12033_2014_9797_MOESM1_ESM.pdf (2.7 mb)
Supplementary material 1 (PDF 2721 kb)

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Ehsan Pourabed
    • 1
  • Farzan Ghane Golmohamadi
    • 1
  • Peyman Soleymani Monfared
    • 1
  • Seyed Morteza Razavi
    • 1
  • Zahra-Sadat Shobbar
    • 1
    Email author
  1. 1.Systems Biology DepartmentAgricultural Biotechnology Research Institute of Iran (ABRII)KarajIran

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