Molecular Biotechnology

, Volume 56, Issue 2, pp 175–191 | Cite as

Statistical Screening of Medium Components for Recombinant Production of Pseudomonas aeruginosa ATCC 9027 Rhamnolipids by Nonpathogenic Cell Factory Pseudomonas putida KT2440

  • Payam Setoodeh
  • Abdolhossein Jahanmiri
  • Reza Eslamloueyan
  • Ali Niazi
  • Seyyed Shahaboddin Ayatollahi
  • Farzaneh Aram
  • Maziyar Mahmoodi
  • Ali Hortamani
Research

Abstract

Rhamnolipids (RLs) produced by the opportunistic human pathogen Pseudomonas aeruginosa are considered as potential candidates for the next generation of surfactants. Large-scale production of RLs depends on progress in strain engineering, medium design, operating strategies, and purification procedures. In this work, the rhlAB genes extracted from a mono_RLs_producing strain of P. aeruginosa (ATCC 9027) were introduced to an appropriate safety host Pseudomonas putida KT2440. The capability of the recombinant strain was evaluated in various media. As a prerequisite for optimal medium design, a set of 32 experiments was performed in two steps for screening a number of macro-nutritional compounds. In the experiments, a two-level fractional factorial design resolution IV was followed by a two-level full factorial one. By means of this approach, it was observed that glycerol, yeast extract, and peptone have significant positive influence on recombinant RLs production while the yeast extract/peptone two-factor and glycerol/yeast extract/peptone three-factor interactions have considerable negative effects. A wide range of variation from 0 to 570 mg/l was obtained for RLs production during the screening experiments indicating the importance of medium optimization. The results point out the opportunity for possible higher yields of RLs through further screening, mixture/combined mixture designs, and high-cell-density cultivations.

Keywords

Heterologous rhamnolipids production Pseudomonas aeruginosa ATCC 9027 Pseudomonas putida KT2440 Transformation Experimental screening design Two-level factorial design 

Notes

Acknowledgments

The authors would like to express their deepest gratitude to Professor Victor de Lorenzo, Centro Nacional de Biotecnología, CSIC Madrid 28049, Spain, for providing pVLT33 plasmid and Professor Ibrahim Banat, School of Biomedical Sciences, University of Ulster, County Londonderry, Northern Ireland, for providing the concentrated crude RLs solution. The authors also wish to thank the reviewers for their valuable comments, which have contributed to the improvement of the manuscript.

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Payam Setoodeh
    • 1
  • Abdolhossein Jahanmiri
    • 1
  • Reza Eslamloueyan
    • 1
  • Ali Niazi
    • 2
  • Seyyed Shahaboddin Ayatollahi
    • 3
    • 4
  • Farzaneh Aram
    • 2
  • Maziyar Mahmoodi
    • 1
  • Ali Hortamani
    • 2
  1. 1.School of Chemical and Petroleum EngineeringShiraz UniversityShirazIran
  2. 2.Institute of BiotechnologyShiraz UniversityShirazIran
  3. 3.EOR Research CenterShiraz UniversityShirazIran
  4. 4.Sharif University of TechnologyTehranIran

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