Molecular Biotechnology

, Volume 54, Issue 2, pp 320–330

The Choline Oxidase Gene codA Confers Salt Tolerance to Transgenic Eucalyptus globulus in a Semi-Confined Condition

  • Xiang Yu
  • Akira Kikuchi
  • Etsuko Matsunaga
  • Yoshihiko Morishita
  • Kazuya Nanto
  • Nozomu Sakurai
  • Hideyuki Suzuki
  • Daisuke Shibata
  • Teruhisa Shimada
  • Kazuo N. Watanabe
Research

DOI: 10.1007/s12033-012-9575-y

Cite this article as:
Yu, X., Kikuchi, A., Matsunaga, E. et al. Mol Biotechnol (2013) 54: 320. doi:10.1007/s12033-012-9575-y

Abstract

The performance of tree species is influenced by environmental factors and growth stages. To evaluate the practical performance of transgenic tree species, it is insufficient to grow small, young trees under controlled conditions, such as in a growth chamber. Three transgenic Eucalyptus globulus lines, carrying the choline oxidase gene, were investigated for their salt tolerance and expression of the transgene at the young plantlet stage in a special netted-house. To clarify the characteristics at the young as well during the later stages, salt tolerance and the properties of the transgenic lines at large juvenile and adult stages were evaluated in the special netted-house. All transgenic lines showed high glycinebetaine content, particularly in young leaves. Trees of the transgenic line 107-1 showed low damage because of salinity stress based on the results from the chlorophyll analysis and malondialdehyde content, and they survived the high-salt-shock treatment at the large juvenile and adult stages. Only this line showed salt tolerance at all stages in the special netted-house. In this evaluation in the special netted-house, the tolerant line among young plantlets might perform better at all stages. Since evaluation in these special netted-house mimics field evaluation, line 107-1 is a potential tolerant line.

Keywords

Eucalyptus globulus Salt tolerance Choline oxidase Semi-confined condition Special netted-house 

Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Xiang Yu
    • 1
    • 4
  • Akira Kikuchi
    • 1
  • Etsuko Matsunaga
    • 2
  • Yoshihiko Morishita
    • 3
    • 5
  • Kazuya Nanto
    • 2
  • Nozomu Sakurai
    • 3
  • Hideyuki Suzuki
    • 3
  • Daisuke Shibata
    • 3
  • Teruhisa Shimada
    • 2
  • Kazuo N. Watanabe
    • 1
  1. 1.Graduate School of Life and Environmental SciencesUniversity of TsukubaTsukubaJapan
  2. 2.Forestry Science LaboratoryNippon Paper Industries Co., LtdTokyoJapan
  3. 3.Kazusa DNA Research InstituteChibaJapan
  4. 4.RIKENYokohamaJapan
  5. 5.Bruker Dbltonics K.KYokohamaJapan

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