Molecular Biotechnology

, Volume 53, Issue 2, pp 150–158 | Cite as

Molecular Studies on the Transmission of Indian Cassava Mosaic Virus (ICMV) and Sri Lankan Cassava Mosaic Virus (SLCMV) in Cassava by Bemisia tabaci and Cloning of ICMV and SLCMV Replicase Gene from Cassava

  • Raghu Duraisamy
  • Senthil NatesanEmail author
  • Raveendran Muthurajan
  • Karthikayan Gandhi
  • Pugalendhi Lakshmanan
  • Nageswari Karuppusamy
  • Mohan Chokkappan


Cassava mosaic disease, caused by cassava mosaic geminiviruses are transmitted by Bemisia tabaci. The B. tabaci adults from colonies reared on virus free cassava plant produced from apical meristem culture was studied to determine their ability to transmit Indian cassava mosaic virus (ICMV) and Sri Lankan cassava mosaic virus (SLCMV) from cassava to cassava. Virus free plants were confirmed by polymerase chain reaction (PCR) using geminivirus degenerate primers. The virus acquisition access period (AAP) of 48 h on virus infected cassava leaves and 48 h virus inoculation access periods on virus free healthy leaves were investigated. Both ICMV and SLCMV were absolutely transmitted by whiteflies reared on cassava. Virus specific primers were designed in the replicase region and used to detect virus in B. tabaci after different AAP. The PCR amplified replicase genes from virus transmitted cassava leaves were cloned the plasmid DNA was isolated from a recombinant colony of E. coli DH5α after their confirmation by colony PCR and sequenced them. The nucleotide sequences obtained from automated DNA sequencing were confirmed as ICMV and SLCMV replicase gene after homology searching by BLAST and found to be a new isolates. The nucleotide sequences of new isolates were submitted in GenBank (accession number JN652126 and JN595785).


B. tabaci Cloning Meristem culture Sequencing Virus transmission 



Acquisition access period


African cassava mosaic disease


African cassava mosaic virus




Base pair


Basic local alignment search tool




Cassava mosaic disease


Cassava mosaic geminiviruses


Cassava mosaic virus


Deoxy ribonucleic acid


East African cassava mosaic Cameroon virus


East African cassava mosaic Kenya virus


East African cassava mosaic Malawi virus


East African cassava mosaic virus


East African cassava mosaic Zanzibar virus


Ethidium bromide


Gibberellic acid


Inoculation access period


Indian cassava mosaic virus














National center for biotechnology information




South African cassava mosaic virus


Sodium dodecyl sulfate


Sri Lankan cassava mosaic virus


Murashige and Skoog


Meristem culture medium


1-naphthalene acetic acid


Polymerase chain reaction


Relative humidity


Tamil Nadu Agricultural University



The authors would like to thank Rajiv Gandhi National Fellowship (RGNF) grant (No: F. 14-2 (SC)/2008 (SA-III) Dt: 14.09.2009) to Mr. D. Raghu from the University Grant Commission (UGC), New Delhi, India for financial support. We express our sincere thanks to Dr. K. Shanmugam, Professor and Head for providing CMD infected cassava stakes maintained at Tapioca and Castor Research Station (TCRS), Yethapur at Salem in Tamil Nadu. We are also grateful to the Professor and head, Department of Plant Molecular Biology and Biotechnology (DPMBB), Centre for Plant Molecular Biology (CPMB), Tamil Nadu Agricultural University (TNAU) for providing the laboratory facilities.


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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Raghu Duraisamy
    • 1
  • Senthil Natesan
    • 1
    Email author
  • Raveendran Muthurajan
    • 1
  • Karthikayan Gandhi
    • 2
  • Pugalendhi Lakshmanan
    • 3
  • Nageswari Karuppusamy
    • 4
  • Mohan Chokkappan
    • 5
  1. 1.Department of Plant Biotechnology, Centre for Plant Molecular Biology and BiotechnologyTamil Nadu Agricultural UniversityCoimbatoreIndia
  2. 2.Centre for Plant Protection StudiesTamil Nadu Agricultural UniversityCoimbatoreIndia
  3. 3.Horticulture College and Research InstituteTamil Nadu Agricultural UniversityCoimbatoreIndia
  4. 4.Tapioca and Castor Research StationTamil Nadu Agricultural UniversitySalemIndia
  5. 5.Central Tuber Crops Research InstituteTrivandrumIndia

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