Molecular Biotechnology

, Volume 52, Issue 2, pp 170–179

High Yield of Human Monoclonal Antibody Produced by Stably Transfected Drosophila Schneider 2 Cells in Perfusion Culture Using Wave Bioreactor

  • Lulan Wang
  • Hongxing Hu
  • Jianjun Yang
  • Feng Wang
  • Christian Kaisermayer
  • Paul Zhou
Research

Abstract

Since it was first introduced in late 1990s Wave bioreactor has been used for protein production by mammalian and insect cell lines. However, using Wave bioreactor to produce human monoclonal antibody by stable Drosophila Schneider 2 (S2) cell transfectants has not been reported before. In this study, S2 cells were co-transfected with an inducible vector expressing human monoclonal antibody heavy and light chains, respectively, specific for hemagglutinin (HA) of H5N1 influenza virus. Stable S2 transfectant clone was selected by limiting dilution assay. Stable S2 transfectant clone that produce the highest amount of human monoclonal antibody was inoculated into two 2-l disposable cellbags, where cell growth and antibody production were compared between batch and perfusion cultures using Wave bioreactor. Here, we report that maximum viable cell density reached 1.06 × 107 cells/ml in batch culture; whereas 1.04 × 108 cells/ml was achieved in perfusion culture. The maximum volumetric antibody productivity in batch culture was 52 mg/l/day; while perfusion culture yielded 1,437 mg/l/day. As a result, the total antibody production was 201 mg in batch culture and 8,212 mg in perfusion culture. The antibody produced by both cultures displays full neutralizing activity. Thus, our results provide strong support for using Wave bioreactor in perfusion culture for a large-scale production of human monoclonal antibody by stable S2 cell transfectants.

Keywords

Human monoclonal antibody Drosophila S2 cells Wave bioreactor Perfusion Influenza virus 

Supplementary material

12033_2011_9484_MOESM1_ESM.doc (81 kb)
Supplementary material 1 (DOC 81 kb)

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Lulan Wang
    • 1
  • Hongxing Hu
    • 1
  • Jianjun Yang
    • 2
  • Feng Wang
    • 1
  • Christian Kaisermayer
    • 3
  • Paul Zhou
    • 1
  1. 1.Unit of Anti-Viral Immunity and Genetic Therapy, Key Laboratory of Molecular Virology and ImmunologyInstitut Pasteur of Shanghai, Chinese Academy of SciencesShanghaiChina
  2. 2.Fast Trak CenterGE (China) Research and Development Center Co., Ltd.ShanghaiChina
  3. 3.Fast Trak CenterGE Healthcare (Europe) Institute of Applied MicrobiologyWienAustria

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