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Molecular Biotechnology

, Volume 51, Issue 3, pp 283–288 | Cite as

Genetic Characterization of Escherichia coli O157:H7 Strains Isolated from the One-Humped Camel (Camelus dromedarius) by Using Microarray DNA Technology

  • Taghi Zahraei Salehi
  • Alfreda Tonelli
  • Alberto Mazza
  • Hamid Staji
  • Pietro Badagliacca
  • Iradj Ashrafi Tamai
  • Reza Jamshidi
  • Josée Harel
  • Rossella Lelli
  • Luke Masson
Research

Abstract

From the Camelidae family members, several serotypes of Escherichia coli (E. coli) have recently been isolated from diarrhoeic and non-diarrhoeic faecal samples. To date Shiga toxin-producing E. coli (STEC) strains have never been typed in one-humped camel (Camelus dromedarius). In the present study, two E. coli O157:H7 strains isolated from sick dromedaries were investigated. Virulence gene profiles were determined using a custom E. coli virulence DNA microarray, composed of 70-mer oligonucleotide probes targeting 264 virulence or related genes of known E. coli pathotypes. Both strains displayed positive hybridization signals for the Locus of enterocyte effacement (LEE) gene probes (ler, eae, espA, espB, tir genes), two Shiga toxin probes (stx1 and stx2), the O157 O-antigen specific probe, various virulence plasmid (pO157) probes like katP in addition to other accessory virulence genes characterized in STEC.

Keywords

Camelidae Camelus dromedarius Escherichia coli O157:H7 EHEC Microarray STEC 

Notes

Acknowledgment

We thank Dr. Armando Giovannini of Istituto G. Caporale, Epidemiology unit, for his suggestions.

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Taghi Zahraei Salehi
    • 1
  • Alfreda Tonelli
    • 2
  • Alberto Mazza
    • 3
  • Hamid Staji
    • 4
  • Pietro Badagliacca
    • 2
  • Iradj Ashrafi Tamai
    • 1
  • Reza Jamshidi
    • 4
  • Josée Harel
    • 5
  • Rossella Lelli
    • 2
  • Luke Masson
    • 3
  1. 1.Department of Microbiology, Faculty of Veterinary MedicineUniversity of TehranTehranIran
  2. 2.Istituto “G. Caporale”, Campo BoarioTeramoItaly
  3. 3.Biotechnology Research InstituteNational Research Council of CanadaMontrealCanada
  4. 4.Department of Microbiology, Faculty of Veterinary MedicineUniversity of SemnanSemnanIran
  5. 5.Groupe de Recherche sur les Maladies Infectieuses du Porc, Faculté de Médecine VétérinaireUniversité de MontréalMontrealCanada

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