Genetic Diversity of Fusarium oxysporum f.sp. cubense Isolates (Foc) of India by Inter Simple Sequence Repeats (ISSR) Analysis
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To find out the genetic diversity of Indian Foc isolates of banana, a total of 107 isolates of Fusarium which includes 98 Foc isolates obtained from different banana growing regions of India and seven Foc isolates belong to all known VCGs obtained from Australia and two non-pathogenic Fusarium oxysporum (npFo) isolates were subjected to ISSR analysis. In the initial screening of ISSR primers, out of 34, 10 primers which generated more polymorphic bands were selected for further analysis. The Phylogenetic analysis carried out based on the fingerprints obtained through ISSR analysis indicated the presence of wide genetic diversity among the Foc isolates of India and also its polyphyletic nature. Totally, seven different clusters were obtained and these clusters differentiated the Foc isolates of India based on the races/VCGs. Besides, the cluster analysis clearly distinguished the freshly emerged Foc strain obtained from cv. Grand Naine (Cavendish-AAA) and Poovan (Mysore-AAB) from the other Foc isolates. The non-pathogenic F. oxysporum isolates which have been included for comparison purpose also clustered separately. All these above said findings indicates for the first time the discriminatory power of ISSR to clearly distinguish and separate the Foc isolates according to its race/VCGs and also its virulence. This study would be useful not only to design and develop effective management strategies but also useful for quarantine purposes.
KeywordsISSR analysis Fusarium wilt of banana Diversity analysis
We thank Mr. V. Govindasamy, Senior Research Fellow for his technical assistance and Dr. Lisa Maree Gulino, QDPI &F, Brisbane for the supply of DNA samples of Fusarium spp.
- 2.Thangavelu, R., & Mustaffa, M. M. (2010). First report on the occurrence of a virulent strain of Fusarium wilt pathogen (Race-1) infecting Cavendish (AAA) group of bananas in India. Plant Disease 94, doi: 10.1094/PDIS-05-10-0330.
- 4.Leong, S. K., Latiffah, Z., & Baharuddin, S. (2010). Genetic diversity of Fusarium oxysporum f.sp. cubense isolates from Malaysia. African Journal of Microbiological Research, 4, 1026–1037.Google Scholar
- 5.Ploetz, R. C. (1990). Population biology of Fusarium oxysporum f. sp. cubense. In R. C. Ploetz (Ed.), Fusarium wilt of banana (pp. 63–76). St. Paul: The American Phytopathological Society.Google Scholar
- 7.Ainsworth, G. C. (1971). Ainsworth and bisbys dictionary of fungi (6th ed.). Kew, Surrey: Common Wealth Mycological Institute.Google Scholar
- 8.Ricker, A. J., & Ricker, R. S. (1936). Introduction to research in plant diseases. New York: John Swift.Google Scholar
- 9.Carlier, J., De Wale, D., & Escalant, J. V. (2002). Global evaluation of Musa germplasm for resistance of Fusarium wilt, Mycosphaerella leaf spot diseases and nematodes. Technical Guidelines No.6. France: International Network for the improvement of Banana and Plantain.Google Scholar
- 12.Hampl, V., Pavlicek, A., & Flegr, J. (2001). Construction and bootstrap analysis of DNA fingerprinting-based phylogenetic trees with the freeware program FreeTree: application to trichomonad parasites. International Journal of Systematic and Evolutionary Microbiology, 51, 731–735.CrossRefGoogle Scholar
- 13.Sneath, P. H., & Sokal, R. R. (1973). Numerical taxonomy. San Francisco: W. H. Freeman & Co.Google Scholar
- 14.Moore, N. Y. (1994). Fusarium wilt of banana: Pathogen variability and host pathogen interaction. Ph.D. thesis. The University of Queensland, Brisbane.Google Scholar
- 15.Jones, D. R. (2000). Diseases of banana, abacá and enset. Wallingford: CABI Publishing.Google Scholar
- 16.Raguchander, T., Jayashree, K., & Samiyappan, R. (1997). Management of Fusarium wilt of banana using antagonistic microorganisms. Journal of Biological Control, 11, 101–105.Google Scholar
- 23.Kumar, B., Udaya Shankar, A. C., Chandra Nayaka, S., Ranachandra Kini, S., Shetty, H. S., & Prakash, H. S. (2010). Biochemical characterization of Fusarium oxysporum f.sp. cubense isolates from India. African Journal of Biotechnology, 9, 523–530.Google Scholar