Molecular Biotechnology

, Volume 50, Issue 2, pp 145–158

Magnaporthe oryzae Populations Adapted to Finger Millet and Rice Exhibit Distinctive Patterns of Genetic Diversity, Sexuality and Host Interaction

  • J. P. Takan
  • J. Chipili
  • S. Muthumeenakshi
  • N. J. Talbot
  • E. O. Manyasa
  • R. Bandyopadhyay
  • Y. Sere
  • S. K. Nutsugah
  • P. Talhinhas
  • M. Hossain
  • A. E. Brown
  • S. Sreenivasaprasad
Research

Abstract

In this study, host-specific forms of the blast pathogen Magnaporthe oryzae in sub-Saharan Africa (SSA) were characterised from distinct cropping locations using a combination of molecular and biological assays. Finger millet blast populations in East Africa revealed a continuous genetic variation pattern and lack of clonal lineages, with a wide range of haplotypes. M. oryzae populations lacked the grasshopper (grh) element (96%) and appeared distinct to those in Asia. An overall near equal distribution (47–53%) of the mating types MAT1-1 and MAT1-2, high fertility status (84–89%) and the dominance of hermaphrodites (64%) suggest a strong sexual reproductive potential. Differences in pathogen aggressiveness and lack of cultivar incompatibility suggest the importance of quantitative resistance. Rice blast populations in West Africa showed a typical lineage-based structure. Among the nine lineages identified, three comprised ~90% of the isolates. Skewed distribution of the mating types MAT1-1 (29%) and MAT1-2 (71%) was accompanied by low fertility. Clear differences in cultivar compatibility within and between lineages suggest R gene-mediated interactions. Distinctive patterns of genetic diversity, sexual reproductive potential and pathogenicity suggest adaptive divergence of host-specific forms of M. oryzae populations linked to crop domestication and agricultural intensification.

Keywords

Finger millet Rice Blast disease Magnaporthe oryzae Pathogen populations Genetic diversity Mating type distribution Fertility status Pathogenicity Adaptive divergence 

Supplementary material

12033_2011_9429_MOESM1_ESM.doc (1.5 mb)
Supplementary material 1 (DOC 1530 kb)

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • J. P. Takan
    • 1
    • 2
  • J. Chipili
    • 1
    • 3
  • S. Muthumeenakshi
    • 1
  • N. J. Talbot
    • 4
  • E. O. Manyasa
    • 5
  • R. Bandyopadhyay
    • 6
  • Y. Sere
    • 7
  • S. K. Nutsugah
    • 8
  • P. Talhinhas
    • 1
    • 9
  • M. Hossain
    • 1
    • 10
  • A. E. Brown
    • 11
  • S. Sreenivasaprasad
    • 1
    • 12
  1. 1.University of WarwickCoventryUK
  2. 2.NaSARRISorotiUganda
  3. 3.Ministry of AgricultureLusakaZambia
  4. 4.University of ExeterExeterUK
  5. 5.ICRISATNairobiKenya
  6. 6.IITAIbadanNigeria
  7. 7.ARC-WARDACotonouBenin
  8. 8.SARITamaleGhana
  9. 9.CIFC/IICTOeirasPortugal
  10. 10.BRRIGazipurBangladesh
  11. 11.Queen’s UniversityBelfastUK
  12. 12.Institute of Research in Applied Natural Sciences (LIRANS), Division of Science, Faculty of Creative Arts, Technologies and ScienceUniversity of BedfordshireLutonUK

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