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Molecular Biotechnology

, Volume 48, Issue 3, pp 244–254 | Cite as

Clones Identification and Genetic Characterization of Garnacha Grapevine by Means of Different PCR-Derived Marker Systems

  • Stefano MeneghettiEmail author
  • Angelo Costacurta
  • Enrica Frare
  • Graziana Da Rold
  • Daniele Migliaro
  • Giacomo Morreale
  • Manna Crespan
  • Vicente Sotés
  • Antonio Calò
Research

Abstract

This study uses PCR-derived marker systems to investigate the extent and distribution of genetic variability of 53 Garnacha accessions coming from Italy, France and Spain. The samples studied include 28 Italian accessions (named Tocai rosso in Vicenza area; Alicante in Sicily and Elba island; Gamay perugino in Perugia province; Cannonau in Sardinia), 19 Spanish accessions of different types (named Garnacha tinta, Garnacha blanca, Garnacha peluda, Garnacha roja, Garnacha erguida, Garnacha roya) and 6 French accessions (named Grenache and Grenache noir). In order to verify the varietal identity of the samples, analyses based on 14 simple sequence repeat (SSR) loci were performed. The presence of an additional allele at ISV3 locus (151 bp) was found in four Tocai rosso accessions and in a Sardinian Cannonau clone, that are, incidentally, chimeras. In addition to microsatellite analysis, intravarietal variability study was performed using AFLP, SAMPL and M-AFLP molecular markers. AFLPs could discriminate among several Garnacha samples; SAMPLs allowed distinguishing few genotypes on the basis of their geographic origin, whereas M-AFLPs revealed plant-specific markers, differentiating all accessions. Italian samples showed the greatest variability among themselves, especially on the basis of their different provenance, while Spanish samples were the most similar, in spite of their morphological diversity.

Keywords

Cannonau Grenache AFLP M-AFLP SAMPL Intravarietal variability 

Notes

Acknowledgment

Authors thank Franco E., Lorente M., Nuñez R. for providing Spanish Garnacha clones.

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Stefano Meneghetti
    • 1
    Email author
  • Angelo Costacurta
    • 1
  • Enrica Frare
    • 1
  • Graziana Da Rold
    • 1
  • Daniele Migliaro
    • 1
  • Giacomo Morreale
    • 1
  • Manna Crespan
    • 1
  • Vicente Sotés
    • 2
  • Antonio Calò
    • 1
  1. 1.C.R.A.-VIT Consiglio per la ricerca e la sperimentazione in agricolturaCentro di ricerca per la viticolturaConegliano (TV)Italy
  2. 2.Universidad Politécnica de Madrid. ETSI Agrónomos, Ciudad UniversitariaMadridSpain

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