Molecular Biotechnology

, Volume 48, Issue 2, pp 109–115

Enhanced CHO Cell-Based Transient Gene Expression with the Epi-CHO Expression System

  • Joe Codamo
  • Trent P. Munro
  • Benjamin S. Hughes
  • Michael Song
  • Peter P. Gray
Research

DOI: 10.1007/s12033-010-9351-9

Cite this article as:
Codamo, J., Munro, T.P., Hughes, B.S. et al. Mol Biotechnol (2011) 48: 109. doi:10.1007/s12033-010-9351-9

Abstract

Transient gene expression systems in mammalian cells continue to grow in popularity due to their capacity to produce significant amounts of recombinant protein in a rapid and scalable manner, without the lengthy time periods and resources required for stable cell line development. Traditionally, production of recombinant monoclonal antibodies for pre-clinical assessment by transient expression in CHO cells has been hampered by low titers. In this report, we demonstrate transient monoclonal antibody titers of 140 mg/l with CHO cells using the episomal-based transient expression system, Epi-CHO. Such titers were achieved by implementing an optimized transfection protocol incorporating mild-hypothermia and through screening of a variety of chemically defined and serum-free media for their ability to support elevated and prolonged viable cell densities post-transfection, and in turn, improve recombinant protein yields. Further evidence supporting Epi-CHO’s capacity to enhance transgene expression is provided, where we demonstrate higher transgene mRNA and protein levels of two monoclonal antibodies and a destabilized enhanced green fluorescent protein with Epi-CHO compared to cell lines deficient in plasmid DNA replication and/or retention post-transfection. The results demonstrate the Epi-CHO system’s capacity for the rapid production of CHO cell-derived recombinant monoclonal antibodies in serum-free conditions.

Keywords

Transient gene expression CHO Monoclonal antibodies Episomal Mammalian cell culture Biopharmaceutical 

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Joe Codamo
    • 1
    • 2
  • Trent P. Munro
    • 1
    • 2
  • Benjamin S. Hughes
    • 1
  • Michael Song
    • 1
  • Peter P. Gray
    • 1
    • 2
  1. 1.The University of Queensland, Australian Institute for Bioengineering and NanotechnologyBrisbaneAustralia
  2. 2.Acyte Biotech Pty LtdBrisbaneAustralia

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