Molecular Biotechnology

, Volume 41, Issue 3, pp 236–246

Comparison of Caspase Genes for the Induction of Apoptosis Following Gene Delivery

Research

Abstract

The polycation poly(ethylenimine) (PEI) was used to deliver the plasmids coding for various combinations of caspases to Cox-2 overexpressing cancer cell lines. It was found that the expression of the delivered genes, controlled by the Cox-2 promoter, correlated with the expression of the endogenous Cox-2 gene in each cell line in a relatively linear manner. Among the various caspase combination regimens, the combination of caspase 3 plus caspase 9 proved to be the most effective because of an apparent synergy between the two gene products, and produced phosphatidylserine flipping in addition to fragmentation of genomic DNA. Caspase 1 appeared to work independently of either caspases 3 or 9, as no synergistic effect was observed. Transfections with genes coding for granzyme B and caspase 8 yielded a lesser amount of cell death. The delivery of a combination of caspase genes could be readily moved to in vivo research of bladder and colon cancer treatments, and holds great applicability to a wide array of additional tumor types.

Keywords

Gene delivery Expression-targeting Caspase Cox-2 Apoptosis Phosphatidylserine 

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Copyright information

© Humana Press 2008

Authors and Affiliations

  1. 1.Laboratory for Gene Therapy and Cellular Engineering, Department of Chemical and Biomolecular EngineeringTulane UniversityNew OrleansUSA
  2. 2.Department of ChemistryGrambling State UniversityGramblingUSA

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