Molecular Biotechnology

, Volume 41, Issue 2, pp 145–151 | Cite as

Molecular and Pathogenic Variation Identified Among Isolates of Corynespora cassiicola

  • Yanxiang Qi
  • Yixian Xie
  • Xin Zhang
  • Jinji Pu
  • Huiqiang Zhang
  • Siliang Huang
  • He Zhang


Pathogenicity tests and inter simple sequence repeat (ISSR) molecular fingerprinting markers were utilized to analyze 24 Corynespora cassiicola isolates obtained from a lot of Hevea clones grown in most rubber nurseries and a few plantations in China. The C. cassiicola isolates were collected from Hainan and Yunnan provinces, China, from 2006 to 2008. The assay of 24 C. cassiicola isolates on detached leaves of four different Hevea rubber clones (genotypes PR 107, Dafeng 95, RRIM 600, and Reyan 7-33-97) indicated that 23 of the isolates were susceptible to RRIM 600, and were therefore considered race 1 except for CC-023. ISSR analysis grouped 24 C. cassiicola isolates into four clusters (A, B, C, and D). Unweighted pair-group method with arithmetic averaging (UPGMA) analysis based on Nei and Li’s coefficient (calculated from the binary matrix data of 103 DNA fragments generated from 16 ISSR primers) indicated that cluster A included 19 isolates from Hainan and Yunnan (this cluster was further divided into two sub clusters (I, II), sub cluster II contained isolate CC-023); clusters B and C comprised of 1 isolates from Hainan, respectively; while cluster D encompassed 3 isolates from Hainan and Yunnan. Pathogenicity tests and ISSR analysis showed that there was no correlation between race structure, the geographical origin of the pathogen and their ISSR clusters because 23 of the isolates belonging to four distinct clusters were considered race 1 except for isolate CC-023. However, most of the isolates with different pathogenicity levels shared the same clades, and furthermore, the ISSR clusters and cology color had an exact correlation. These results should facilitate the development of rubber clones with enhanced resistance against all genetic clusters of C. cassiicola.


Genetic diversity Corynespora cassiicola Pathogenicity Inter simple sequence repeats (ISSR) 



The research was supported by the grant nyhyzx07-033 from the Ministry of Agriculture, the People’s Republic of China.


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Copyright information

© Humana Press 2008

Authors and Affiliations

  • Yanxiang Qi
    • 1
    • 2
  • Yixian Xie
    • 2
  • Xin Zhang
    • 2
  • Jinji Pu
    • 2
  • Huiqiang Zhang
    • 2
  • Siliang Huang
    • 1
    • 3
  • He Zhang
    • 2
  1. 1.College of Life Science and TechnologyGuangxi UniversityNanningPeople’s Republic of China
  2. 2.Hainan Key Laboratory of Monitoring and Management of Pest of Tropical AgricultureEnvironment and Plant Protection Institute, Chinese Academy of Tropical Agricultural SciencesDanzhouPeople’s Republic of China
  3. 3.Nanyang Normal UniversityNanyangPeople’s Republic of China

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