Molecular Biotechnology

, Volume 39, Issue 2, pp 97–104

Rational Vector Design for Efficient Non-viral Gene Delivery: Challenges Facing the Use of Plasmid DNA


DOI: 10.1007/s12033-008-9046-7

Cite this article as:
Mairhofer, J. & Grabherr, R. Mol Biotechnol (2008) 39: 97. doi:10.1007/s12033-008-9046-7


Although non-viral gene delivery is a very straightforward technology, there are currently no FDA-approved gene medicinal products available. Therefore, improving potency, safety, and efficiency of current plasmid DNA vectors will be a major task for the near future. This article will provide an overview on factors influencing production yield and quality as well as safety issues that emerge from the vector design itself. Special focus will be on generating bacterial pDNA vectors by circumventing the use of antibiotic resistance genes, to generate safer gene medicinal products as well as smaller, more efficient DNA vectors.


Gene delivery Non-viral Transient protein expression Vector design Gene therapy DNA vaccines Plasmid DNA DNA structure Plasmid size Bacterial minimal vector Antibiotic resistance gene 

Copyright information

© Humana Press 2008

Authors and Affiliations

  1. 1.Department of BiotechnologyUniversity of Natural Resources and Applied Life SciencesViennaAustria

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