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Medical Oncology

, 33:119 | Cite as

How strong is the association between IPF and lung cancer? An answer from airway’s DNA

  • G. E. CarpagnanoEmail author
  • D. Lacedonia
  • P. Soccio
  • I. Caccavo
  • G. Patricelli
  • M. P. Foschino Barbaro
Short Communication

Abstract

Idiopathic pulmonary fibrosis is a chronic progressive disease of lung interstitium of unknown etiology with poor prognosis. In patients with IPF, the incidence of lung cancer is much higher than that in the general population. The identification of noninvasive biomarkers for early diagnosis of IPF is of great relevance in consideration of the management of these patients. Among the noninvasive omic markers, an increasing interest has been directed toward the study of genetic alterations of microsatellites (MAs) in exhaled breath condensate (EBC). The aim of this preliminary study was to investigate the MAs, located in chromosomal regions 8p21.3–q11.1 and 17q11.2–q21, that harbor tumor suppressor genes, in EBC and in the paired whole blood (WB) of IPF patients. Eleven IPF patients were compared with 10 healthy control subjects. All subjects underwent collection of the EBC and WB. The EBC was collected using a condenser. Four microsatellite markers (THRA1, D17S579, D17S250 and D8S137) were used for the analysis of MAs. The EBC-DNA and WB-DNA were amplified by PCR; PCR products were analyzed using the ABI Prism 310 DNA. Microsatellite alterations were found in 58.82 % of EBC-DNA and 12.50 % of WB-DNA in patients with IPF (p < 0.01). None of the healthy subjects exhibited MAs in the studied markers. Our findings suggest that these genetic alterations, studied in EBC, may play an important role in the complex genetic basis of IPF. Since these MAs are frequently detected in cancer, they might explain the higher relative risk of tumorigenesis in this disease.

Keywords

Microsatellite alterations Lung cancer IPF EBC 

Notes

Author contributions

GEC and PS designed the study; DL, PS, IC and GP contributed to the clinical and laboratory work for the study; GEC took responsibility for the integrity of the data in the study and the accuracy of the data analysis; GEC and PS analyzed the data and wrote paper; GEC, DL and MPFB contributed to critical review and final approval of the manuscript. All authors read and approved the final manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Informed consent

Informed consent was obtained from all individual participants included in the study.

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • G. E. Carpagnano
    • 1
    Email author
  • D. Lacedonia
    • 1
  • P. Soccio
    • 1
  • I. Caccavo
    • 1
  • G. Patricelli
    • 1
  • M. P. Foschino Barbaro
    • 1
  1. 1.Department of Medical and Surgical Sciences, Institute of Respiratory DiseasesUniversity of FoggiaFoggiaItaly

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