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Medical Oncology

, 32:97 | Cite as

Wnt2 protein plays a role in the progression of pancreatic cancer promoted by pancreatic stellate cells

  • Yong Xu
  • Hua Li
  • Chongbiao Huang
  • Tiansuo Zhao
  • Huan Zhang
  • Chen Zheng
  • He Ren
  • Jihui HaoEmail author
Original Paper

Abstract

This study aimed to investigate the expression of Wnt2 protein in pancreatic cancer tissues and pancreatic stellate cells (PSCs), and determine its effect on the biological functions of pancreatic cancer cells. Immunohistochemistry was used to study the expression pattern of Wnt2 in pancreatic cancer tissues. The relationship between Wnt2 protein expression level and patient prognosis was analyzed. PSCs were isolated and cultured. The expression of Wnt2 in activated PSCs was investigated using Western blot and immunofluorescence. We also analyzed the effect of Wnt2 recombinant protein and stellate cell culture supernatant on the Wnt/β-catenin signaling pathway, as well as the effect of Wnt2 recombinant protein on the biological functions of pancreatic cancer cells. The expression of Wnt2 in interstitial cells of pancreatic cancer was correlated with the prognosis of pancreatic cancer. Wnt2 protein was expressed in activated PSCs. Both stellate cell culture supernatant and Wnt2 recombinant protein could activate the classic Wnt/β-catenin signaling pathway. Wnt2 protein enhanced the migration, invasion, and metastasis of pancreatic cancer cells. These results suggested that Wnt2 protein secreted by PSCs promoted the progression of pancreatic cancer by activating the classic Wnt/β-catenin signaling pathway.

Keywords

Pancreatic cancer Stellate cells Wnt signal pathway Wnt2 Epithelial–mesenchymal transition 

Notes

Acknowledgments

This work was supported by grants from the Tianjin City High School Science & Technology Fund Planning Project (No. 20130122).

Conflict of interest

The authors indicated no potential conflicts of interest.

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Yong Xu
    • 1
  • Hua Li
    • 2
  • Chongbiao Huang
    • 3
  • Tiansuo Zhao
    • 4
  • Huan Zhang
    • 4
  • Chen Zheng
    • 4
  • He Ren
    • 4
  • Jihui Hao
    • 4
    • 5
    Email author
  1. 1.Department of Diagnostic and Therapeutic Ultrasonography, Tianjin Medical University Cancer Institute and HospitalNational Clinical Research Center of Cancer, Key Laboratory of Cancer Prevention and TherapyTianjinChina
  2. 2.Department of Endoscopy, Tianjin Medical University Cancer Institute and HospitalNational Clinical Research Center of Cancer, Key Laboratory of Cancer Prevention and TherapyTianjinChina
  3. 3.Department of Senior Wards, Tianjin Medical University Cancer Institute and HospitalNational Clinical Research Center of Cancer, Key Laboratory of Cancer Prevention and TherapyTianjinChina
  4. 4.Department of Pancreatic Cancer, Tianjin Medical University Cancer Institute and HospitalNational Clinical Research Center of Cancer, Key Laboratory of Cancer Prevention and TherapyTianjinChina
  5. 5.Department of Pancreatic CancerTianjin Medical University Cancer Institute and HospitalTianjinChina

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