Medical Oncology

, 31:205 | Cite as

Elevated level of microRNA-21 in the serum of patients with colorectal cancer

  • Gholam Basati
  • Amirnader Emami RazaviEmail author
  • Soheila Abdi
  • Asad Mirzaei
Original Paper


In patients with colorectal cancer, circulating micro RNA-21 (miR-21) is overexpressed and may act as a potential diagnostic and prognostic biomarker. In the present study, serum miR-21 level was determined in patients with colorectal cancer and control subjects in an attempt to explore its potential clinical diagnostic and prognostic value. Serum levels of miR–21 were measured in 40 patients with colorectal adenocarcinoma and 40 control subjects using a quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR) assay. Serum miR-21 levels were compared in the colorectal cancer patients and control subjects. Furthermore, the association between serum miR-21 level and the clinical stages of tumors was also examined in the patients. Serum miR-21 level was significantly elevated in colorectal adenocarcinoma patients relative to control subjects (P = 0.0001), and it was revealed as a potential diagnostic biomarker for differentiating the patients from control subjects. Increased levels of serum miR-21 were associated with clinical stages of tumors in the patients (P = 0.01). These results indicated that serum miR-21 levels could serve as a reliable diagnostic and prognostic biomarker for colorectal adenocarcinoma.


Serum miR-21 Colorectal adenocarcinoma Clinical staging qRT-PCR 



This study was supported by the research Grant, EC/92/H/136, from Ilam University of Medical Sciences.

Conflict of interest

The authors declare that there are no financial conflicts of interest.


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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Gholam Basati
    • 1
  • Amirnader Emami Razavi
    • 2
    Email author
  • Soheila Abdi
    • 3
  • Asad Mirzaei
    • 4
  1. 1.Department of Clinical Biochemistry, Faculty of Allied Medical SciencesIlam University of Medical SciencesIlamIran
  2. 2.Iran National Tumor Bank, Cancer InstituteTehran University of Medical SciencesTehranIran
  3. 3.Department of Physics, Safadasht BranchIslamic Azad UniversityTehranIran
  4. 4.Department of Parasitology, Faculty of MedicineIlam University of Medical ScienceIlamIran

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