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Medical Oncology

, 31:784 | Cite as

Promoter methylation status of MGMT, hMSH2, and hMLH1 and its relationship to corresponding protein expression and TP53 mutations in human esophageal squamous cell carcinoma

  • Yaoyao Su
  • Lihong Yin
  • Ran Liu
  • JingYi Sheng
  • Miao Yang
  • Yi Wang
  • Enchun Pan
  • Wei Guo
  • Yuepu Pu
  • Juan Zhang
  • Geyu Liang
Original Paper

Abstract

To determine the relevance of O-6-methylguanine-DNA methyltransferase (MGMT), human mutS homolog 2 (hMSH2), and human mutL homolog 1 (hMLH1) in TP53 mutations in esophageal squamous cell carcinoma, we employed methylation-sensitive high-resolution melting technology and methylation-specific polymerase chain reaction (PCR) to analyze promoter hypermethylation of MGMT, hMSH2, and hMLH1, respectively, in 51 paired tumors and their adjacent normal tissues. The protein expression of the three proteins was also evaluated by Western blot analysis, and the PCR products of TP53, from exon 5 to exon 8, were directly sequenced to measure the mutation spectrum. Esophageal tumor tissues embraced statistically higher MGMT and hMSH2 promoter methylation level than normal tissue. The promoter methylation status of MGMT and hMSH2 corresponds positively with the protein expression level of MGMT and hMSH2. However, such relevance was not found for hMLH1. Furthermore, TP53 mutation status was well associated with MGMT and hMSH2 promoter methylation status, indicating that silencing of the two genes could lead to TP53 mutation in ESCC.

Keywords

Promoter methylation TP53 mutation Human esophageal squamous cell carcinoma 

Notes

Acknowledgments

This study was funded by the National Natural Science Foundation of China (Nos. 81072259, 30671732, 30800891), Research Fund for the Doctoral Program of Higher Education of China (No. 200802861045), Natural Science Foundation of JiangSu province of China (No. BK2010407), and Research and Teaching Fund for the Excellent Youth Scholars of Southeast University (2009).

Conflict of interest

None.

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Yaoyao Su
    • 1
  • Lihong Yin
    • 1
  • Ran Liu
    • 1
  • JingYi Sheng
    • 1
  • Miao Yang
    • 1
  • Yi Wang
    • 2
  • Enchun Pan
    • 2
  • Wei Guo
    • 3
  • Yuepu Pu
    • 1
  • Juan Zhang
    • 1
  • Geyu Liang
    • 1
  1. 1.Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public HealthSoutheast UniversityNanjingChina
  2. 2.Disease Control and Prevention Center of HuaianHuaianChina
  3. 3.The First People’s Hospital of HuaianHuaianChina

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