Medical Oncology

, 30:483

FISH analysis for TET2 deletion in a cohort of 362 Brazilian myeloid malignancies: correlation with karyotype abnormalities

  • Fábio Morato de Oliveira
  • Carlos Eduardo Miguel
  • Antônio Roberto Lucena-Araujo
  • Ana Silvia Gouvêa de Lima
  • Roberto Passetto Falcão
  • Eduardo Magalhães Rego
Original Paper
  • 301 Downloads

Abstract

We investigated the prevalence of TET2 deletion by using a new FISH probe in a cohort of 362 Brazilian patients with myeloid neoplasms and their association with cytogenetic information (G-banding analysis). Normal karyotype was observed in 45.8 % of MDS (n = 44), 43.8 % of AML (n = 39) and 46.3 % of MPN (n = 82). Abnormalities of 4q24 (deletions, translocations or inversions) were associated with another chromosomal abnormality in four patients by G-banding analysis (2 MDS, 1 AML and 1 MPN). Interphase FISH analysis revealed deletion of TET2 in 21 patients (6 patients with abnormal karyotype and in 15 patients with normal karyotype). arrayCGH analysis revealed a cryptic deletion of the region 4q24 in all eight patients selected with myeloid malignancies (3 MDS, 1 AML and 4 MPN). Considering the significantly high cost of determining the mutational status of TET2 in patient samples by using conventional sequencing methods and sometimes the lack of regular use of SNP/aCGH array methodologies, FISH for the detection of TET2 abnormalities may become a potentially useful clinical tool. The search for alterations in TET2 gene may be important for the prediction of prognosis in normal/altered AML patients’ karyotype or in the disease evolution of patients with MNP and MDS.

Keywords:

TET2 MDS AML MPN Cytogenetics FISH SKY arrayCGH 

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Fábio Morato de Oliveira
    • 1
    • 2
  • Carlos Eduardo Miguel
    • 3
  • Antônio Roberto Lucena-Araujo
    • 1
    • 2
  • Ana Silvia Gouvêa de Lima
    • 1
    • 2
  • Roberto Passetto Falcão
    • 1
    • 2
  • Eduardo Magalhães Rego
    • 1
    • 2
  1. 1.Department of Internal Medicine, Division of Hematology, School of Medicine of Ribeirão PretoUniversity of São PauloRibeirão PretoBrazil
  2. 2.National Institute of Science and Technology in Stem Cell and Cell TherapyRibeirão PretoBrazil
  3. 3.Hematology DivisionHospital of Base of São José do Rio PretoSão PauloBrazil

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