Medical Oncology

, Volume 29, Issue 3, pp 1928–1934 | Cite as

Genetic variants of MnSOD and GPX1 and susceptibility to bladder cancer in a Turkish population

  • Canan Kucukgergin
  • Oner Sanli
  • Akın S. Amasyalı
  • Tzevat Tefik
  • Sule Seckin
Original Paper


This study was conducted to investigate the association of genetic polymorphisms in the MnSOD and GPX1 genes with the risk and invasiveness of bladder cancer in a Turkish population. This prospectively designed study enrolled 157 patients with bladder cancer (mean age 63.2 ± 10.86 years) and 224 healthy controls (mean age 61.7 ± 8.39 years). Genotyping of the MnSOD Ala-9Val and GPX1 Pro198Leu polymorphisms was carried out by PCR–RFLP. No significant difference was found in MnSOD genotype distributions between the controls and the bladder cancer patients. However, the Leu/Leu genotype of GPX1 was associated with a significantly higher risk of bladder cancer than the Pro/Pro genotype. When stratified according to tumor stage, the Leu/Leu genotype of GPX1 was more frequently observed in bladder cancer patients with high-stage tumors than those with low-stage tumors. Additionally, patients carrying both Ala/Ala of MnSOD and Leu/Leu of GPX1 had the highest risk of developing bladder cancer. In conclusion, the present study indicates that the GPX1 Pro198Leu polymorphism may be associated with the risk and development of invasive bladder cancer. In addition, the combination of the MnSOD Ala/Ala and GPX1 Leu/Leu genotypes may have a synergistic effect on disease risk.


Manganese superoxide dismutase Glutathione peroxidase 1 Single nucleotide polymorphism Polymerase chain reaction–restriction fragment length polymerase analysis Bladder cancer Turkish population 



This work was supported by the Research Fund of Istanbul University, project number: 11658.


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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Canan Kucukgergin
    • 1
  • Oner Sanli
    • 2
  • Akın S. Amasyalı
    • 3
  • Tzevat Tefik
    • 2
  • Sule Seckin
    • 1
  1. 1.Department of Biochemistry, Istanbul Faculty of MedicineIstanbul UniversityIstanbulTurkey
  2. 2.Department of Urology, Istanbul Faculty of MedicineIstanbul UniversityIstanbulTurkey
  3. 3.Department of UrologyIstanbul Teaching and Research HospitalIstanbulTurkey

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