Medical Oncology

, Volume 29, Issue 1, pp 77–83 | Cite as

Upregulated INHBA expression is associated with poor survival in gastric cancer

  • Quan Wang
  • Yu-Gang Wen
  • Da-Peng Li
  • Jun Xia
  • Chong-Zhi Zhou
  • Dong-Wang Yan
  • Hua-Mei Tang
  • Zhi-Hai Peng
Original Paper

Abstract

Expression microarrays are widely used for investigating the candidate molecular targets in human cancer. While genome-wide expression signatures screened by gene set enrichment analysis (GSEA) were not performed in Chinese gastric cancer (GC). To gain new molecular targets for GC, GSEA analysis was performed. In the present study, GSEA were used to pick out differentially expressed gene sets of our database. Total RNA of paired tissue samples (n = 48) and a tissue microarray containing 132 paired tissues were used to further validate expression levels of INHBA and its correction with clinicopathological factors. Upregulated INHBA expression in gastric cancer was screened and further confirmed by qPCR and immunostaining analysis. Increased INHBA expression was significantly correlated with the diameter of cancer and depth of tumor invasion. Patients with higher expression levels of INHBA had a shorter disease-free survival rate. It was effective to gain new molecular targets for GC by GSEA analysis. INHBA may be a poor survival indicator of GC.

Key words

Microarray Gastric cancer Marker Enrichment Prognosis 

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Quan Wang
    • 1
  • Yu-Gang Wen
    • 1
  • Da-Peng Li
    • 1
  • Jun Xia
    • 1
  • Chong-Zhi Zhou
    • 1
  • Dong-Wang Yan
    • 1
  • Hua-Mei Tang
    • 2
  • Zhi-Hai Peng
    • 1
  1. 1.Department of General Surgery, Shanghai First People’s Hospital, College of MedicineShanghai Jiao Tong UniversityShanghaiChina
  2. 2.Department of Pathology, Shanghai First People’s Hospital, College of MedicineShanghai Jiao Tong UniversityShanghaiChina

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