Medical Oncology

, Volume 29, Issue 1, pp 324–331 | Cite as

Vasculogenic mimicry–potential target for glioblastoma therapy: an in vitro and in vivo study

  • Yinsheng Chen
  • Zhitao Jing
  • Cheng Luo
  • Minghua Zhuang
  • Junzhe Xia
  • Zhongping Chen
  • Yunjie WangEmail author
Original Paper


Glioblastoma is one of the most angiogenic human tumors and characterized by microvascular proliferations. A better understanding of glioblastoma vasculature is needed to optimize anti-angiogenic therapy that has shown a promising but incomplete efficacy. The present study examined 48 glioblastomas by CD34 endothelial marker periodic acid–Schiff (PAS) dual staining and found non-endothelial cell-lined blood vessels that were formed by tumor cells (vasculogenic mimicry, VM) existing in a fraction of these tumors. We hypothesized that CD133-positive glioblastoma stem-like cells (GSCs) may play a pivotal role in glioblastoma VM formation and then demonstrated in vitro and in vivo that a subset of GSCs were capable of vasculogenesis. Moreover, we found that several growth factors involved in normal angiogenesis were expressed in GSCs. We describe here a new mechanism of alternative glioblastoma vascularization and open a new perspective for the anti-vascular treatment strategy.


Glioblastoma Angiogenesis Vasculogenic mimicry Stem cell 



We wish to thank Xi-meng Yin and Lei Cui Ph.D., for their excellent technical assistance.


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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Yinsheng Chen
    • 1
  • Zhitao Jing
    • 1
  • Cheng Luo
    • 2
  • Minghua Zhuang
    • 2
  • Junzhe Xia
    • 1
  • Zhongping Chen
    • 3
    • 4
  • Yunjie Wang
    • 1
    Email author
  1. 1.Department of Neurosurgery, The First Affiliated HospitalChina Medical UniversityShenyangPeople’s Republic of China
  2. 2.Department of Neurosurgery, The First Affiliated HospitalShantou University Medical CollegeShantouPeople’s Republic of China
  3. 3.State Key Laboratory for Cancer Research in Southern ChinaSun Yat-sen UniversityGuangzhouPeople’s Republic of China
  4. 4.Department of Neurosurgery/Neuro-oncology, Cancer CenterSun Yat-sen UniversityGuangzhouPeople’s Republic of China

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