Identification of candidate molecular markers of nasopharyngeal Carcinoma by tissue microarray and in situ hybridization
- 316 Downloads
To scan differentially expressed genes and to identify candidate molecular markers in nasopharyngeal carcinoma (NPC), we analyzed cDNA microarray data by GenMAPP to find specifically expressed genes in NPC and used tissue microarray and in situ hybridization techniques to confirm our microarray results. Our cDNA microarray results showed that TSPAN-1 and DPP10 genes were down-expressed in NPC, and COX7B and RFC2 genes were over-expressed in NPC. Real-time quantitative reverse transcription-PCR and in situ hybridization (ISH) techniques confirmed that TSPAN-1 and DPP10 genes had only 40.72 and 40.70% positive expression in NPC, but had high positive expression in chronic inflammation of nasopharyngeal mucosa (P < 0.01). However, COX7B and RFC2 genes were high positive rate in NPC (84.24 and 64.53%, respectively) than in normal control tissues. The data suggested that TSPAN-1, DPP10, COX7B and RFC2 genes might be the putative molecular markers of NPC.
KeywordsNasopharyngeal carcinoma (NPC) Tissue microarray In situ hybridization Molecular marker
In situ hybridization
Cytochrome c oxidase subunit VIIb
Replication factor C (activator 1) 2
H2A histone family, member Z
Chaperonin containing TCP1, subunit 2 (beta)
Interferon-induced protein 44
APEX nuclease (multifunctional DNA repair enzyme) 1
General transcription factor IIE, polypeptide 2, beta 34 kDa
Myosin light chain kinase
Zinc finger CCCH-type containing 11A
Immunoglobulin J polypeptide, linker protein for immunoglobulin alpha and mu polypeptides
Tripartite motif-containing 26
Mitochondrial ribosomal protein S18B
Aryl-hydrocarbon receptor nuclear translocator 2
SH3 domain-binding glutamic acid-rich protein
Chemokine (C–C motif) receptor 9
Armadillo repeat containing, X-linked 6
The authors thank Dr. Ming Li for guidance in data analysis.
- 11.Chrysovergis A, Gorgoulis VG, Giotakis I, et al. Simultaneous over activation of EGFR, telomerase (h TERT), and cyclin D1 correlates with advanced disease in larynx squamous cell carcinoma: a tissue microarray analysis. Med Oncol. 2010. doi: 10.1007/s12032-010-9522-3.
- 12.Xue Y-J, Lu Q, Sun Z-X. CD147 overexpression is a prognostic factor and a potential therapeutic target in bladder cancer. Med Oncol. 2010. doi: 10.1007/s12032-010-9582-4.
- 15.Shanmugaratnam K, Sobin LH. Histological typing of tumours of the upper respiratory tract and ear. 2nd ed. Berlin; New York: Springer; 1991.Google Scholar
- 17.Liu ZQ, Tian YQ, Hu YF, Li XL, Ma FR, Li GY. Alteration of gene expression during nasopharyngeal carcinogenesis revealed by oligonucleotide microarray after microdissection of tumor tissue and normal epithelia from nasopharynx. Chin Med J (Engl). 2009;122(4):437–43.Google Scholar