Medical Oncology

, Volume 28, Supplement 1, pp 528–539

Down-regulation of HPV18 E6, E7, or VEGF expression attenuates malignant biological behavior of human cervical cancer cells

Original Paper

DOI: 10.1007/s12032-010-9690-1

Cite this article as:
Chen, L., Wu, YY., Liu, P. et al. Med Oncol (2011) 28(Suppl 1): 528. doi:10.1007/s12032-010-9690-1

Abstract

To investigate the effect of down-regulation of VEGF (vascular endothelial growth factor) and HPV18 E6/E7 by hairpin RNA (shRNA) on cell proliferation, apoptosis, migration, invasion, and adhesion abilities of cervical carcinoma cells, recombinant plasmids including pS-E6 shRNA, pS-E7 shRNA, and pS-VEGF shRNA were constructed and transfected into HeLa cells. The levels of E6 mRNA, E7 mRNA, or VEGF mRNA were significantly reduced after transfection of pS-E6 shRNA (76.0%), pS-E7 shRNA (74.4%), and pS-VEGF shRNA (46.7%). VEGF expression was down-regulated by pS-E6 shRNA (55.1%) and pS-E7 shRNA (46.6%). The apoptosis of HeLa cells was increased, and the proliferation, invasion, and adhesion abilities were decreased significantly. For in vivo study, cancer cells that stably expressed the plasmids were cultured. Cells were transplanted subcutaneously into nude mice to establish xenograft tumor model. Finally, expression of E6 shRNA, E7 shRNA, and VEGF shRNA in cancer cells led to inhibition of the growth of xenograft. Hence, RNA interference could effectively suppress the expression of HPV18 E6/E7 and VEGF in human cervical cancer cells. This suppression attenuates malignant biological behavior of human cervical cancer cells. RNA interference of HPV E6/E7 or VEGF expression implies an effective anti-cervical cancer strategy.

Keywords

HPV18 E6 E7 oncoproteins VEGF Cervical cancer 

Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Li Chen
    • 1
  • Yuan-Yuan Wu
    • 1
  • Peigen Liu
    • 2
  • Jianli Wang
    • 2
  • Guilan Wang
    • 1
  • Jin Qin
    • 1
  • Jiaming Zhou
    • 1
  • Jianwei Zhu
    • 1
    • 2
  1. 1.Department of PathologyNantong University School of MedicineNantongChina
  2. 2.Medical Center of Nantong University, Affiliated HospitalNantong UniversityNantongChina

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