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Medical Oncology

, Volume 27, Issue 4, pp 1219–1226 | Cite as

Apoptosis of human colorectal carcinoma cells is induced by blocking hepatoma-derived growth factor

  • Fei Liao
  • Weiguo Dong
  • Lifang Fan
Original Paper

Abstract

Hepatoma-derived growth factor (HDGF) is a novel multifunctional growth factor that elicits pleiotropic effects on biological processes such as lung remodeling and renal development. Recent studies demonstrated that HDGF is related to tumor proliferation, invasion, angiogenesis, and apoptosis. However, the molecular mechanism of HDGF’s involvement in apoptosis remains to be clarified. In this study, we first analyze the role of HDGF in colorectal carcinoma (CRC) progression by immunohistochemistry. Then we used small interference RNA (HDGF-siRNA) to block HDGF and assessed its effect on inducing apoptosis of CRC loVo cells. Apoptosis was detected using flow cytometry (FCM), DNA ladder analysis, and Hoechst 33258 staining. In addition, the expression levels of some apoptosis-related proteins were examined by western blot. The result showed that HDGF expression gradually increased in the colorectal carcinogenesis process. Further studies demonstrated that knock-down of HDGF can down-regulate the survivin, activate the mitochondrial pathway, and induce apoptosis in loVo cells. These findings suggest that HDGF is involved in colorectal carcinogenesis process. Further blocking HDGF exhibits potent pro-apoptotic properties in colon cancer cells. Thus, HDGF might be a potential therapeutic target for human colorectal cancer. These findings may have major implications in the treatment of colorectal cancer.

Keywords

Hepatoma-derived growth factor Apoptosis Colorectal cancer Mitochondrial pathway 

Notes

Acknowledgments

This study was supported by a grant from the Natural Science Foundation of Hubei Province, China (project no. 2007AA301B33-3), as well as by the Medical Structural Biology Research Center of Wuhan University.

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Copyright information

© Humana Press Inc. 2009

Authors and Affiliations

  1. 1.Department of GastroenterologyRenmin Hospital of Wuhan UniversityWuhanPeople’s Republic of China
  2. 2.Pathology DepartmentWuhan UniversityWuhanPeople’s Republic of China

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