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Medical Oncology

, Volume 27, Issue 4, pp 1102–1108 | Cite as

Upregulation of soluble resistance-related calcium-binding protein (sorcin) in gastric cancer

  • Langmei Deng
  • Tao Su
  • Aimin Leng
  • Xiaomei Zhang
  • Meihua xu
  • Lu Yan
  • Huan Gu
  • Guiying Zhang
Original Paper

Abstract

The aim of this paper was to identify novel proteins involved in the development of gastric cancer (GC). Isobaric tags for relative and absolute quantification (iTRAQ) analysis was adopted to separate the differentially expressed proteins between normal gastric epithelial cell line GES-1 and GC cell line SGC7901. Western blotting was utilized to validate the increased expression of sorcin in SGC7901; immunohistochemistry was performed to investigate its relationship with clinicopathological features of GC. Twelve differential proteins were identified. Seven proteins were found to be significantly upregulated (≥twofold), while five proteins were markedly downregulated (≤0.5-fold), in SGC7901 cells. Sorcin was detected by this proteomic approach with a 5.4-fold upregulation in SGC7901. Western blotting and immunohistochemistry confirmed the overexpression of sorcin in GC. Immunohistochemistry showed us that sorcin was overexpressed in 55 samples of GC tissue (55/85, 64.71%) and was related closely to the depth of invasion, TNM stage, and lymph node metastasis of GC (P < 0.05). The development of GC is regulated by multiple genes. Sorcin will be a novel molecular biomarker for the diagnosis, treatment, and prognosis of GC.

Keywords

Gastric cancer Sorcin iTRAQ Mass spectrometry Biomarker 

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Copyright information

© Humana Press Inc. 2009

Authors and Affiliations

  • Langmei Deng
    • 1
  • Tao Su
    • 2
  • Aimin Leng
    • 1
  • Xiaomei Zhang
    • 1
  • Meihua xu
    • 1
  • Lu Yan
    • 1
  • Huan Gu
    • 1
  • Guiying Zhang
    • 1
  1. 1.Department of Gastroenterology, Xiangya HospitalCentral South UniversityChangshaPeople’s Republic of China
  2. 2.The Medical Research Center of Xiangya HospitalCentral South UniversityChangshaPeople’s Republic of China

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