Medical Oncology

, Volume 27, Issue 4, pp 1096–1101 | Cite as

Cell cycle inhibition and apoptosis induced by curcumin in Ewing sarcoma cell line SK-NEP-1

  • Mansher Singh
  • Ambarish Pandey
  • Collins A. Karikari
  • Gayatri Singh
  • Dinesh Rakheja
Original Paper


Curcumin is a naturally occurring polyphenolic compound found in the turmeric, which is used as food additive in Indian cooking and as a therapeutic agent in traditional Indian medicine. Curcumin is currently under investigation as a chemotherapeutic and chemopreventive agent in adult cancer models at both pre-clinical and clinical levels. In this preliminary study, we show that curcumin is effective in causing cell cycle arrest, inducing apoptosis, and suppressing colony formation in the Ewing sarcoma cell line SK-NEP-1. Curcumin causes upregulation of cleaved caspase 3 and downregulation of phospho-Akt, producing apoptosis in Ewing sarcoma cells at an inhibitory concentration 50% (IC50) of approximately 4 μM. Our findings indicate a need for further evaluation of curcumin in chemotherapy and chemoprevention of Ewing sarcoma.


Curcumin Ewing sarcoma Apoptosis 



The authors thank Dr Anirban Maitra, Gan M. Zou, and Richard L. Boriack for their constant support and guidance. We would also like to thank Dr. George Feldmann and Dr. Savita Bisht for their help at different stages of the project.


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Copyright information

© Humana Press Inc. 2009

Authors and Affiliations

  • Mansher Singh
    • 1
  • Ambarish Pandey
    • 1
  • Collins A. Karikari
    • 2
  • Gayatri Singh
    • 1
  • Dinesh Rakheja
    • 3
    • 4
  1. 1.All India Institute of Medical SciencesNew DelhiIndia
  2. 2.Department of Pathology, The Sol Goldman Pancreatic Cancer Research CenterJohns Hopkins University School of MedicineBaltimoreUSA
  3. 3.Department of PathologyChildren’s Medical CenterDallasUSA
  4. 4.University of Texas Southwestern Medical CenterDallasUSA

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