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Medical Oncology

, Volume 25, Issue 3, pp 341–345 | Cite as

Expression and clinical significance of NET-1 and PCNA in hepatocellular carcinoma

  • Shi-Qiang ShenEmail author
  • Kun Li
  • Neng Zhu
  • Akimasa Nakao
Original Paper

Abstract

Objective Objective of this study was to detect the expression of neuroepithelial transforming gene-1 (NET-1) and proliferating cell nuclear antigen (PCNA) in hepatocellular carcinoma (HCC) and adjacent tissues, and to investigate the relation of the expression of NET-1 in HCC tissue with cancer proliferation, metastasis and clinic stages. Methods The expression of NET-1 mRNA was detected by reverse transcription-polymerase chain reaction method in 34 human HCC tissues, and it was matched with 34 paracarcinoma tissues. The expression of PCNA in HCC was analyzed by immunohistochemistry. Meanwhile, the relation of the expression with clinic pathological features of HCC was evaluated, and the correlation between the expression of NET-1 and PCNA in HCC was investigated. Results Expression of NET-1 was significantly higher in HCC than that in matched paracarcinoma tissues. The expression of NET-1 was significantly higher in TMN III–IV HCC tissues when compared with TMN I–II HCC tissues (P < 0.05). The expression level of NET-1 in HCC tissues was related to intrahepatic metastasis and portal vein infiltration. The expression of NET-1 in HCC tissues was positively correlated with PCNA. Conclusions The expression of NET-1 may relate to proliferation, metastasis and clinic stages of HCC. The expression of NET-1 in HCC tissues may positively correlate to the TMN stages.

Keywords

NET-1 PCNA Hepatocellular carcinoma 

Notes

Acknowledgments

We appreciate the contributions of Mr. Hong Xia and Ms. Li Yu for their technical support during the experiment at the Research Institute of Gastroenterology & Hepatology of Renmin Hospital of Wuhan University.

References

  1. 1.
    Morgan TR, Mandayam S, Jamal MM. Alcohol and hepatocellular carcinoma. Gastroenterology 2004;127:S87–S96.PubMedCrossRefGoogle Scholar
  2. 2.
    Symons M, Rusk N. Control of vesicular trafficking by Rho GTPases. Curr Biol 2003;13:409–18.CrossRefGoogle Scholar
  3. 3.
    Alberts AS, Treisman R. Activation of RhoA and SAPK/JNK signalling pathways by the RhoA-specific exchange factor mNET1. EMBO J 1998;17:4075–85.PubMedCrossRefGoogle Scholar
  4. 4.
    Wollscheid V, Kuhne-Heid R, Stein I, et al. Identification of a new proliferation-associated protein net-1/c4-8 characteristic for a subset of high-grade cervical intraepithelial neoplasia and cervical carcinoma. Int J Cancer 2002;99(6):771–5.PubMedCrossRefGoogle Scholar
  5. 5.
    Leyden J, Murray D, Moss A, et al. NET-1 and Myeov: computationally identified mediators of gastric cancer. Br J Cancer 2006;94(8):1204–12.PubMedCrossRefGoogle Scholar
  6. 6.
    Lupberger J, Hildt E. Hepatitis B virus-induced oncogenesis. World J Gastroenterol 2007;13(1):74–81.PubMedGoogle Scholar
  7. 7.
    Gobel T, Vorderwulbecke S, Hauck K, Fey H, Haussinger D, Erhardt A. New multi protein patterns differentiate liver fibrosis stages and hepatocellular carcinoma in chronic hepatitis C serum samples. World J Gastroenterol 2006;12(47):7604–12.PubMedGoogle Scholar
  8. 8.
    Minagawa M, Makuuchi M. Treatment of hepatocellular carcinoma accompanied by portal vein tumor thrombus. World J Gastroenterol 2006;12(47):7561–7.PubMedGoogle Scholar
  9. 9.
    Park Y, Chae KJ, Kim YB, et al. Apoptosis and proliferation in hepatocarcinogenesis related to cirrhoisis. Cancer 2001;92(11):2733–8.PubMedCrossRefGoogle Scholar
  10. 10.
    Pizem J, Marolt VF, Luzar B, et al. Proliferative and apoptotic activity in hepatocellular carcinoma and surrounding non-neoplastic liver tissue. Pflugers Arch 2001;442(6 Suppl 1):174–6.CrossRefGoogle Scholar
  11. 11.
    Zeng WJ, Liu GY, Xu J, Zhou XD, Zhang YE, Zhang N. Pathological characteristics, PCNA labeling index and DNA index in prognostic eveluation of patients with oderately differentiated hepatocellular carcinoma. World J Gastroenterol 2002;8:1040–4.PubMedGoogle Scholar
  12. 12.
    Kong XB, Liang LJ, Huang JF. A Study of correlation between apoptosis,expression of p21 protein and PCNA and the clinical prognosis in hepatocellular carcinoma. Aizheng J 1999;18:426–9.Google Scholar
  13. 13.
    Shen LJ, Zhang HX, Zhang ZJ, et al. Detection of HBV, PCNA and GST-pi in hepatocelluar carcinoma and chronic liver diseases. World J Gastroenterol 2003;9(3):459–62.PubMedGoogle Scholar
  14. 14.
    Rossman KL, Der CJ, Sondek J. GEF means go: turning on RHO GTPases with guanine nucleotide-exchange factors. Nat Rev Mol Cell Biol 2005;6:167–80.PubMedCrossRefGoogle Scholar
  15. 15.
    Etienne-Manneville S, Hall A. Rho GTPases in cell biology. Nature 2002;420:629–35.PubMedCrossRefGoogle Scholar
  16. 16.
    Jaffe AB, Hall A. Rho GTPases in transformation and metastasis. Adv Cancer Res 2002;84:57–80.PubMedCrossRefGoogle Scholar
  17. 17.
    Sahai E, Marshall CJ. RHO-GTPases and cancer. Nat Rev Cancer 2002;2:133–42.PubMedCrossRefGoogle Scholar
  18. 18.
    Yauch RL, Hemler ME. Specific interactions among transmembrane 4 superfamily (TM4SF) proteins and phosphoinositide 4-kinase. Biochem J 2000;351:629–37.PubMedCrossRefGoogle Scholar
  19. 19.
    Serru V, Dessen P, Boucheix C, Rubinstein E. Sequence and expression of seven new tetraspans. Biochim Biophys Acta 2000;1478:159–63.PubMedGoogle Scholar
  20. 20.
    Maecker HT, Todd SC, Levy S. The tetraspanin superfamily: molecular facilitators. FASEB J 1997;11(6):428–42.PubMedGoogle Scholar
  21. 21.
    Serru V, Le Naour F, Billard M, et al. Selective tetraspan-integrin complexes (CD81/alpha4beta1, CD151/alpha3beta1, CD151/alpha6beta1) under conditions disrupting tetraspan interactions. Biochem J 1999;340:103–11.PubMedCrossRefGoogle Scholar

Copyright information

© Humana Press Inc. 2008

Authors and Affiliations

  • Shi-Qiang Shen
    • 1
    Email author
  • Kun Li
    • 1
  • Neng Zhu
    • 1
  • Akimasa Nakao
    • 2
  1. 1.Department of General SurgeryRenmin Hospital of Wuhan UniversityWuhanChina
  2. 2.Department of Surgery IINagoya University Graduate School of MedicineNagoyaJapan

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