Epigallocatechin-3-gallate induces apoptosis and cell cycle arrest in HTLV-1-positive and -negative leukemia cells
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The objective of this study is to evaluate the efficacy of epigallocatechin gallate against ATL cells. The anti-proliferative and pro-apoptotic effects of EGCG were evaluated in HTLV-1-positive and -negative cells. EGCG exhibited a marked decrease in proliferation of ATL cells at 96 h of treatment. The results indicated that TGF-α was down-regulated whereas levels of TGF-β2 increased. Cell cycle distribution analysis revealed an increase in cells in the pre-G1 phase which was confirmed by ELISA. The results on proteins showed an up-regulation of p53, Bax and p21 protein levels while the levels of Bcl-2α were down-regulated.
KeywordsApoptosis Human T-cell Lymphotrophic Virus-1 (HTLV-1) Epigallocatechin-3-gallate (EGCG) p53 p21 Transforming Growth Factor (TGF)
Our thanks to the Science Core Laboratory at the American University of Beirut and to Mrs Rania El-osta and Mr. Joe El Khoury for help with the typing and figure editing of this manuscript.
- 1.Hare Y. Green tea: health benefits and applications. New York: Marcel Dekker, Basel; 2001.Google Scholar
- 6.Gupta S, Ahmad N, Nieminen AL, Mukhtar H. Growth inhibition, cell-cycle dysregulation, and induction of apoptosis by green tea constituent (-)-epigallocatechin-3-gallate in androgen-sensitive and androgen-insensitive human prostate carcinoma cells. Toxicol Appl Pharmacol 2000;164:82–90.PubMedCrossRefGoogle Scholar
- 14.Hinuma Y, et al. Transforming genes in human leukemia cells. PNAS 1985;66:1371–8.Google Scholar
- 16.Gessain A. Epidemiology of HTLV-1 and associated diseases. In: Höllsberg P, Hafler DA, editors. Human T-cell lymphotropic virus type 1. John Willey & Sons Ltd.; 1996. p. 33–64.Google Scholar
- 20.Bazarbachi A, Hermine O. Treatment with a combination of zidovudine and alpha-interferon in naïve and pretreated adult T-cell leukemia/lymphoma patients. J Acq Immun Defic Synd Hum 1996;R13(Suppl. 1):5186–90.Google Scholar
- 23.Yang CS, Yang GY, Landau JM, Kim S, Liao J. Exp Lung Res 1998;24:629.Google Scholar
- 29.Saeki K, et al. Cell density-dependent apoptosis in HL-60 cells, which is mediated by an unknown soluble factor, is inhibited by transforming growth factor beta 1 and overexpression of Bcl-2. J Biol Chem 2000;272:3–10.Google Scholar
- 33.Inmann GJ, Allday MJ. Apoptosis induced by TGF-β1 in Burkitt’s Lymphoma cells is caspase 8 dependent but is death receptor independent. J Immunol 2000;165:2500–10.Google Scholar