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Cannabinoid CB2 Receptor Functional Variation (Q63R) Is Associated with Multiple Sclerosis in Iranian Subjects

  • Alireza TahamtanEmail author
  • Shahrzad Rezaiy
  • Saeed Samadizadeh
  • Abdolvahab Moradi
  • Alijan Tabarraei
  • Naeme Javid
  • Morteza Oladnabi
  • Mohammad Hosein Naeimi
Article

Abstract

The cannabinoid system has been identified as a critical endogenous regulator of immune homeostasis through immunomodulatory actions. This system is one of the main regulatory systems of the central nervous system (CNS). Variations in the cannabinoid CB2 receptor gene (CNR2) could affect intracellular signaling and reduce system function, which has been associated with an unbalanced immune response and increased risk of a variety of autoimmune inflammatory disorders. The present study investigated the relationship between CNR2 rs35761398 (Q63R) functional variation and multiple sclerosis (MS). A total of 100 Iranian MS patients and 100 healthy controls were enrolled in the study and genotyped through TaqMan assay. The co-dominant, dominant, recessive, over-dominant, and additive inheritance models were analyzed using SNPStats software. A significant genetic association was observed between Q63R polymorphism and MS. The dominant model was accepted as the best inheritance model to fit the data (OR 2.70, 95% CI 1.47–4.97, p = 0.001). The data implied the involvement of the CNR2 gene in susceptibility to MS in Iranian patients.

Keywords

Cannabinoid receptor type 2 Multiple sclerosis Single-nucleotide polymorphism CNR2 Q63R Iran 

Abbreviations

SNP

single-nucleotide polymorphism

CB2

cannabinoid receptor type 2

ITP

thrombocytopenic purpura

JIA

juvenile idiopathic arthritis

IBD

inflammatory bowel disease

RA

rheumatoid arteritis

MS

multiple sclerosis

OR

odds ratio

CI

confidence interval

HWE

Hardy–Weinberg equilibrium

RRMS

relapsing-remitting MS

SPMS

secondary-progressive MS

PPMS

primary-progressive MS

AIC

Akaike information criterion

CNS

central nerve system

EC

endocannabinoid

Notes

Acknowledgments

The authors would like to thank all the participants in the current study.

Funding Information

This work was financially supported by Golestan University of Medical Sciences (No. 231195).

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.

Ethical Approval

The current human study was approved by the science and bioethics committee of Golestan University of Medical Sciences (IR.GOUMS.REC.1397.045).

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Neuroscience Research CenterGolestan University of Medical SciencesGorganIran
  2. 2.Department of Microbiology, Faculty of MedicineGolestan University of Medical SciencesGorganIran
  3. 3.Islamic Azad University of MashhadMashhadIran
  4. 4.Ischemic Disorders Research CenterGolestan University of Medical SciencesGorganIran
  5. 5.Department of Neurology, Sayyad HospitalGolestan University of Medical SciencesGorganIran

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