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Journal of Molecular Neuroscience

, Volume 69, Issue 4, pp 628–635 | Cite as

LPP and RYR2 Gene Polymorphisms Correlate with the Risk and the Prognosis of Astrocytoma

  • Qu Chen
  • Yao Sun
  • Jiamin Wu
  • Zichao Xiong
  • Fanglin Niu
  • Tianbo JinEmail author
  • Qiujian ZhaoEmail author
Article
  • 47 Downloads

Abstract

Astrocytoma is the most common neuroepithelial tumor. Genetic factors play an important role in the development and prognosis of astrocytoma. So this study focuses on the impact of LPP and RYR2 genes on the occurrence and prognosis of astrocytoma. Rs12594 and rs16835904 in the RYR2 gene and rs1064607, rs3796283, and rs2378456 in the LPP gene were selected and genotyped using Agena MassARRAY in 365 patients and 379 healthy populations. Odds ratios (ORs) and 95% confidence intervals (CIs) were determined using logistic regression to assess the influence of gene polymorphisms on occurrence of astrocytoma. The association between genotype and survival outcomes was performed by the Kaplan-Meier method, the log-rank test, and the Cox regression analysis. The survival rates of patients receiving gross total resection and postoperative chemotherapy were higher than patients receiving near total resection and subtotal resection and without chemotherapy. In recessive model, the patients with LPP rs2378456 CC genotype increased the risk of astrocytoma (OR = 1.43, 95% CI 1.01–2.02, p = 0.042). Stratified analysis shows that RYR2 rs16835904 TC-TT genotype facilitated the risk of astrocytoma in male (OR = 1.93, 95% CI 1.15–3.24, p = 0.011). Cox regression analysis shows that RYR2 rs12594 AA genotype and AG genotype were associated with OS of astrocytoma (AG genotype: HR = 1.62, 95% CI 1.04–2.53, p = 0.034; AA genotype: HR = 1.70, 95% CI 1.08–2.68, p = 0.021). RYR2 and LPP genes were found to affect the occurrence and prognosis of astrocytoma.

Keywords

Astrocytoma RYR2 gene LPP gene Genetic susceptibility Prognosis 

Notes

Acknowledgments

We are grateful to all patients who participated in the study. We would also like to thank the clinicians and other hospital staff who contributed to the data collection for this study.

Author’s Contributions

QC prepared manuscript, YS collected data, JMW and ZCX analyzed data, FLN searched literature, TBJ and QJZ designed the study.

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.

References

  1. Adamec C (1964) Example of the use of the nonparametric test. Test X2 for comparison of 2 independent examples. Cesk Zdrav 12:613–619Google Scholar
  2. Bland JM, Altman DG (2000) Statistics notes. The odds ratio. BMJ 320:1468CrossRefGoogle Scholar
  3. Chandrashekar DS, Bashel B, Balasubramanya SAH, Creighton CJ, Ponce-Rodriguez I, Chakravarthi B, Varambally S (2017) UALCAN: a portal for facilitating tumor subgroup gene expression and survival analyses. Neoplasia 19:649–658.  https://doi.org/10.1016/j.neo.2017.05.002 CrossRefPubMedPubMedCentralGoogle Scholar
  4. Dobrev D, Wehrens XH (2014) Role of RyR2 phosphorylation in heart failure and arrhythmias: controversies around ryanodine receptor phosphorylation in cardiac disease. Circ Res 114:1311–1319. discussion 1319.  https://doi.org/10.1161/CIRCRESAHA.114.300568 CrossRefPubMedPubMedCentralGoogle Scholar
  5. Dong YS, Hou WG, Li XL, Jin TB, Li Y, Feng DY, Liu DB, Gao GD, Yin ZM, Qin HZ (2014) Genetic association of CHEK2, GSTP1, and ERCC1 with glioblastoma in the Han Chinese population. Tumour Biol 35:4937–4941.  https://doi.org/10.1007/s13277-014-1648-z CrossRefPubMedGoogle Scholar
  6. Grunewald TG, Pasedag SM, Butt E (2009) Cell adhesion and transcriptional activity - defining the role of the novel protooncogene LPP. Transl Oncol 2:107–116CrossRefGoogle Scholar
  7. Huang SQ, Zhang N, Zhou ZX, Huang CC, Zeng CL, Xiao D, Guo CC, Han YJ, Ye XH, Ye XG, Ou ML, Zhang BH, Liu Y, Zeng E, Yang G, Jing CX (2017) Association of LPP and TAGAP polymorphisms with celiac disease risk: a meta-analysis. Int J Environ Res Public Health 14.  https://doi.org/10.3390/ijerph14020171 CrossRefGoogle Scholar
  8. Jin L, Kern MJ, Otey CA, Wamhoff BR, Somlyo AV (2007) Angiotensin II, focal adhesion kinase, and PRX1 enhance smooth muscle expression of lipoma preferred partner and its newly identified binding partner palladin to promote cell migration. Circ Res 100:817–825.  https://doi.org/10.1161/01.RES.0000261351.54147.de CrossRefPubMedGoogle Scholar
  9. Jin T et al (2015) Analysis of difference of association between polymorphisms in the XRCC5, RPA3 and RTEL1 genes and glioma, astrocytoma and glioblastoma. Am J Cancer Res 5:2294–2300PubMedPubMedCentralGoogle Scholar
  10. Jin TB et al (2016) Polymorphism in the IL4R gene and clinical features are associated with glioma prognosis: analyses of case-cohort studies. Medicine (Baltimore) 95:e4231.  https://doi.org/10.1097/MD.0000000000004231 CrossRefGoogle Scholar
  11. Keime-Guibert F, Chinot O, Taillandier L, Cartalat-Carel S, Frenay M, Kantor G, Guillamo JS, Jadaud E, Colin P, Bondiau PY, Meneï P, Loiseau H, Bernier V, Honnorat J, Barrié M, Mokhtari K, Mazeron JJ, Bissery A, Delattre JY (2007) Radiotherapy for glioblastoma in the elderly. N Engl J Med 356:1527–1535.  https://doi.org/10.1056/NEJMoa065901 CrossRefPubMedGoogle Scholar
  12. Kuriyama S, Yoshida M, Yano S, Aiba N, Kohno T, Minamiya Y, Goto A, Tanaka M (2016) LPP inhibits collective cell migration during lung cancer dissemination. Oncogene 35:952–964.  https://doi.org/10.1038/onc.2015.155 CrossRefPubMedGoogle Scholar
  13. Leung CS, Yeung TL, Yip KP, Wong KK, Ho SY, Mangala LS, Sood AK, Lopez-Berestein G, Sheng J, STC W, Birrer MJ, Mok SC (2018) Cancer-associated fibroblasts regulate endothelial adhesion protein LPP to promote ovarian cancer chemoresistance. J Clin Invest 128:589–606.  https://doi.org/10.1172/JCI95200 CrossRefPubMedGoogle Scholar
  14. Li Z, Wong KY, Chan GC, Chim CS (2018) Epigenetic silencing of LPP/miR-28 in multiple myeloma. J Clin Pathol 71:253–258.  https://doi.org/10.1136/jclinpath-2017-204501 CrossRefPubMedGoogle Scholar
  15. Li Z, Xu Z, Xie Q, Gao W, Xie J, Zhou L (2016) miR-1303 promotes the proliferation of neuroblastoma cell SH-SY5Y by targeting GSK3beta and SFRP1 biomedicine & pharmacotherapy. Biomed Pharmacother 83:508–513.  https://doi.org/10.1016/j.biopha.2016.07.010 CrossRefPubMedGoogle Scholar
  16. Liu Y, Han L, Bai Y, Du W, Yang B (2018) Down-regulation of MicroRNA-133 predicts poor overall survival and regulates the growth and invasive abilities in glioma. Artif Cells Nanomed Biotechnol 46:206–210.  https://doi.org/10.1080/21691401.2017.1304551 CrossRefGoogle Scholar
  17. Ma Z (2018) Downregulation of SETD8 by miR-382 is involved in glioma progression. Pathol Res Pract 214:356–360.  https://doi.org/10.1016/j.prp.2018.01.004 CrossRefPubMedGoogle Scholar
  18. McGirt MJ et al (2008) Extent of surgical resection is independently associated with survival in patients with hemispheric infiltrating low-grade gliomas. Neurosurgery 63:700–707. author reply 707-708.  https://doi.org/10.1227/01.neu.0000325729.41085.73 CrossRefPubMedGoogle Scholar
  19. Petit MM, Meulemans SM, Van de Ven WJ (2003) The focal adhesion and nuclear targeting capacity of the LIM-containing lipoma-preferred partner (LPP) protein. J Biol Chem 278:2157–2168.  https://doi.org/10.1074/jbc.M206106200 CrossRefPubMedGoogle Scholar
  20. Tang Z, Li C, Kang B, Gao G, Li C, Zhang Z (2017) GEPIA: a web server for cancer and normal gene expression profiling and interactive analyses. Nucleic Acids Res 45:W98–W102.  https://doi.org/10.1093/nar/gkx247 CrossRefPubMedPubMedCentralGoogle Scholar
  21. Thomas RK, Baker AC, DeBiasi RM, Winckler W, LaFramboise T, Lin WM, Wang M, Feng W, Zander T, MacConaill LE, Lee JC, Nicoletti R, Hatton C, Goyette M, Girard L, Majmudar K, Ziaugra L, Wong KK, Gabriel S, Beroukhim R, Peyton M, Barretina J, Dutt A, Emery C, Greulich H, Shah K, Sasaki H, Gazdar A, Minna J, Armstrong SA, Mellinghoff IK, Hodi FS, Dranoff G, Mischel PS, Cloughesy TF, Nelson SF, Liau LM, Mertz K, Rubin MA, Moch H, Loda M, Catalona W, Fletcher J, Signoretti S, Kaye F, Anderson KC, Demetri GD, Dummer R, Wagner S, Herlyn M, Sellers WR, Meyerson M, Garraway LA (2007) High-throughput oncogene mutation profiling in human cancer. Nat Genet 39:347–351.  https://doi.org/10.1038/ng1975 CrossRefPubMedGoogle Scholar
  22. Wick W, Platten M, Meisner C, Felsberg J, Tabatabai G, Simon M, Nikkhah G, Papsdorf K, Steinbach JP, Sabel M, Combs SE, Vesper J, Braun C, Meixensberger J, Ketter R, Mayer-Steinacker R, Reifenberger G, Weller M, NOA-08 Study Group of Neuro-oncology Working Group (NOA) of German Cancer Society (2012) Temozolomide chemotherapy alone versus radiotherapy alone for malignant astrocytoma in the elderly: the NOA-08 randomised, phase 3 trial. Lancet Oncol 13:707–715.  https://doi.org/10.1016/s1470-2045(12)70164-x CrossRefGoogle Scholar
  23. Wulff H, Kolski-Andreaco A, Sankaranarayanan A, Sabatier JM, Shakkottai V (2007) Modulators of small- and intermediate-conductance calcium-activated potassium channels and their therapeutic indications. Curr Med Chem 14:1437–1457CrossRefGoogle Scholar
  24. Xiong J, Liu X, Gong Y, Zhang P, Qiang S, Zhao Q, Guo R, Qian Y, Wang L, Zhu L, Wang R, Hao Z, Wen H, Zhang J, Tang K, Zang WF, Yuchi Z, Chen H, Chen SRW, Zheng W, Wang SQ, Xu YW, Liu Z (2018) Pathogenic mechanism of a catecholaminergic polymorphic ventricular tachycardia causing-mutation in cardiac calcium release channel RyR2. J Mol Cell Cardiol 117:26–35.  https://doi.org/10.1016/j.yjmcc.2018.02.014 CrossRefPubMedGoogle Scholar
  25. Xu D, Ma P, Gao G, Gui Y, Niu X, Jin B (2015) MicroRNA-383 expression regulates proliferation, migration, invasion, and apoptosis in human glioma cells. Tumour Biol 36:7743–7753.  https://doi.org/10.1007/s13277-015-3378-2 CrossRefPubMedGoogle Scholar
  26. Yeh PS, Wu SJ, Hung TY, Huang YM, Hsu CW, Sze CI, Hsieh YJ, Huang CW, Wu SN (2016) Evidence for the inhibition by temozolomide, an imidazotetrazine family alkylator, of intermediate-conductance Ca2+-activated K+ channels in glioma cells. Cell Physiol Biochem 38:1727–1742.  https://doi.org/10.1159/000443112 CrossRefPubMedGoogle Scholar
  27. Zhang B, Zhao H, Li T, Gao X, Gao Q, Tang R, Zhang J, Chen ZJ (2012) Association study of gene LPP in women with polycystic ovary syndrome. PLoS One 7:e46370.  https://doi.org/10.1371/journal.pone.0046370 CrossRefPubMedPubMedCentralGoogle Scholar
  28. Zhang J, Chen B, Zhong X, Mi T, Guo A, Zhou Q, Tan Z, Wu G, Chen AW, Fill M, Song LS, Chen SRW (2014) The cardiac ryanodine receptor luminal Ca2+ sensor governs Ca2+ waves, ventricular tachyarrhythmias and cardiac hypertrophy in calsequestrin-null mice. Biochem J 461:99–106.  https://doi.org/10.1042/BJ20140126 CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Clinical LaboratoryBaoji Central HospitalBaojiChina
  2. 2.Key Laboratory of Resource Biology and Biotechnology in Western China (Northwest University), Ministry of EducationNorthwest UniversityXi’anChina

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