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RNA Sequencing of Peripheral Blood Revealed that the Neurotropic TRK Receptor Signaling Pathway Shows Apparent Correlation in Recovery Following Spinal Cord Injury at Small Cohort

  • Chunqing WangEmail author
  • Hangzhou Lv
  • Qing LiEmail author
  • Ke Gong
  • Lei Luo Yang
  • Zean Wei
  • Yujie Pan
  • Mingyong Wang
Article
  • 1 Downloads

Abstract

Spinal cord injury (SCI) can be lethal; however, the precise mechanisms underlying healing are unclear, limiting the development of effective therapies. In this study, the molecular mechanisms involved in SCI were investigated. Clinical peripheral blood samples from normal individuals and patients with incomplete SCI (ISCI) and complete SCI (CSCI) were analyzed by RNA-Seq. The expression levels of EPHA4, CDK16, BAD, MAP2 Normal 2, EGR, and RHOB differed significantly between the SCI group and normal individuals, and these results were verified by q-PCR. A gene ontology (GO) enrichment analysis showed that differentially expressed genes were mostly enriched for the neurotrophin TRK receptor signaling pathway. We verified the expression of neurotrophic factors and found that they were all expressed most highly in the SCI group. The results of this study demonstrate that neurotrophic factors are highly expressed after SCI and the neurotrophin TRK receptor signaling pathway may be involved in the initiation of nerve system regeneration.

Keywords

Peripheral blood RNA sequencing Neurotrophin TRK receptor signaling pathway Spinal cord injury 

Notes

Funding Information

This work was supported by grants from the Guiyang Science and Technology Bureau fund (2018-1-88).

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Orthopedic Traumathe Affiliated Hospital of Guizhou Medical UniversityGuiyang CityChina
  2. 2.Department of RehabilitationJizhong Energy Xingtai MIG General HospitalXingtai CityChina
  3. 3.Department of Orthopedicsthe Second Affiliated Hospital of Soochow UniversitySuzhouChina

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