Advertisement

Aberrant Expression of Long Non-coding RNAs in Peripheral Blood of Autistic Patients

  • Arezou Sayad
  • Mir Davood Omrani
  • Hamid Fallah
  • Mohammad Taheri
  • Soudeh Ghafouri-Fard
Article
  • 34 Downloads

Abstract

Long non-coding RNAs (lncRNAs) constitute a large proportion of human transcriptome and are involved in fundamental aspects of neurodevelopment. In the present study, we evaluated expression levels of three lncRNAs, namely, Nuclear Paraspeckle Assembly Transcript 1 (NEAT1), p21-associated ncRNA DNA damage–activated (PANDA), and taurine-upregulated gene 1 (TUG1), in peripheral blood of autism spectrum disorder (ASD) patients compared with those in healthy subjects. We found a significant upregulation of NEAT1 and TUG1 in ASD patients. In addition, relative expressions of the pairs of lncRNAs were significantly correlated in both patients and healthy subjects. However, expressions of these lncRNAs were not correlated with age of study participants. The present study provides further evidences for dysregulation of lncRNAs in ASD patients.

Keywords

Autism spectrum disorder NEAT1 PANDA TUG1 lncRNA 

Notes

Funding Information

The current study was supported by a grant from Shahid Beheshti University of Medical Sciences.

References

  1. Association AP (2013) Diagnostic and statistical manual of mental disorders (DSM-5®), American Psychiatric PubGoogle Scholar
  2. Bao MH, Szeto V, Yang BB, Zhu SZ, Sun HS, Feng ZP (2018) Long non-coding RNAs in ischemic stroke. Cell Death Dis 9:281CrossRefGoogle Scholar
  3. Barry G, Guennewig B, Fung S, Kaczorowski D, Weickert CS (2015) Long non-coding RNA expression during aging in the human subependymal zone. Front Neurol 6:45CrossRefGoogle Scholar
  4. Bernard D, Prasanth KV, Tripathi V, Colasse S, Nakamura T, Xuan ZY, Zhang MQ, Sedel F, Jourdren L, Coulpier F, Triller A, Spector DL, Bessis A (2010) A long nuclear-retained non-coding RNA regulates synaptogenesis by modulating gene expression. EMBO J 29:3082–3093CrossRefGoogle Scholar
  5. Clemson CM, Hutchinson JN, Sara SA, Ensminger AW, Fox AH, Chess A, Lawrence JB (2009) An architectural role for a nuclear noncoding RNA: NEAT1 RNA is essential for the structure of paraspeckles. Mol Cell 33:717–726CrossRefGoogle Scholar
  6. Goff LA, Groff AF, Sauvageau M, Trayes-Gibson Z, Sanchez-Gomez DB, Morse M, Martin RD, Elcavage LE, Liapis SC, Gonzalez-Celeiro M, Plana O, Li E, Gerhardinger C, Tomassy GS, Arlotta P, Rinn JL (2015) Spatiotemporal expression and transcriptional perturbations by long noncoding RNAs in the mouse brain. Proc Natl Acad Sci U S A 112:6855–6862CrossRefGoogle Scholar
  7. Gottfried C, Bambini-Junior V, Francis F, Riesgo R, Savino W (2015) The impact of neuroimmune alterations in autism spectrum disorder. Front Psychiatry 6:121CrossRefGoogle Scholar
  8. Gudenas BL, Srivastava AK, Wang L (2017) Integrative genomic analyses for identification and prioritization of long non-coding RNAs associated with autism. PLoS One 12:e0178532CrossRefGoogle Scholar
  9. Hung T, Wang YL, Lin MF, Koegel AK, Kotake Y, Grant GD, Horlings HM, Shah N, Umbricht C, Wang P, Wang Y, Kong B, Langerod A, Borresen-Dale AL, Kim SK, Van De Vijver M, Sukumar S, Whitfield ML, Kellis M, Xiong Y, Wong DJ, Chang HY (2011) Extensive and coordinated transcription of noncoding RNAs within cell-cycle promoters. Nat Genet 43:621–U196CrossRefGoogle Scholar
  10. Li XJ (2018) Long non-coding RNA nuclear paraspeckle assembly transcript 1 inhibits the apoptosis of retina Muller cells after diabetic retinopathy through regulating miR-497/brain-derived neurotrophic factor axis. Diab Vasc Dis Res 15:204–213CrossRefGoogle Scholar
  11. Peng W, Wang Z, Fan H (2017) LncRNA NEAT1 impacts cell proliferation and apoptosis of colorectal cancer via regulation of Akt signaling. Pathol Oncol Res 23:651–656CrossRefGoogle Scholar
  12. Saghazadeh A, Rezaei N (2017) Brain-derived neurotrophic factor levels in autism: a systematic review and meta-analysis. J Autism Dev Disord 47:1018–1029CrossRefGoogle Scholar
  13. Shi Z, Piccus Z, Zhang X, Yang H, Jarrell H, Ding Y, Teng Z, Tchernichovski O, Li X (2018) miR-9 regulates basal ganglia-dependent developmental vocal learning and adult vocal performance in songbirds. Elife 7Google Scholar
  14. Stoner R, Chow ML, Boyle MP, Sunkin SM, Mouton PR, Roy S, Wynshaw-Boris A, Colamarino SA, Lein ES, Courchesne E (2014) Patches of disorganization in the neocortex of children with autism. N Engl J Med 370:1209–1219CrossRefGoogle Scholar
  15. Taheri M, Omrani MD, Ghafouri-Fard S (2018) Long non-coding RNA expression in bladder cancer. Biophys Rev 10:1205–1213CrossRefGoogle Scholar
  16. Tang J, Yu Y, Yang W (2017) Long noncoding RNA and its contribution to autism spectrum disorders. CNS Neurosci Ther 23:645–656CrossRefGoogle Scholar
  17. Wang Y, Zhao X, Ju W, Flory M, Zhong J, Jiang S, Wang P, Dong X, Tao X, Chen Q, Shen C, Zhong M, Yu Y, Brown WT, Zhong N (2015) Genome-wide differential expression of synaptic long noncoding RNAs in autism spectrum disorder. Transl Psychiatry 5:e660CrossRefGoogle Scholar
  18. Wei H, Alberts I, Li X (2014) The apoptotic perspective of autism. Int J Dev Neurosci 36:13–18CrossRefGoogle Scholar
  19. Yin KJ, Deng Z, Huang H, Hamblin M, Xie C, Zhang J, Chen YE (2010) miR-497 regulates neuronal death in mouse brain after transient focal cerebral ischemia. Neurobiol Dis 38:17–26CrossRefGoogle Scholar
  20. Yin D-D, Zhang E-B, You L-H, Wang N, Wang L-T, Jin F-Y, Zhu Y-N, Cao L-H, Yuan Q-X, De W (2015) Downregulation of lncRNA TUG1 affects apoptosis and insulin secretion in mouse pancreatic β cells. Cell Physiol Biochem 35:1892–1904CrossRefGoogle Scholar
  21. Yuva-Aydemir Y, Simkin A, Gascon E, Gao FB (2011) MicroRNA-9: functional evolution of a conserved small regulatory RNA. RNA Biol 8:557–564CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Medical GeneticsShahid Beheshti University of Medical SciencesTehranIran
  2. 2.Urogenital Stem Cell Research CenterShahid Beheshti University of Medical SciencesTehranIran
  3. 3.Student Research CommitteeShahid Beheshti University of Medical SciencesTehranIran

Personalised recommendations