An Intraperitoneal Treatment with Calcitonin Gene-Related Peptide (CGRP) Regulates Appetite, Energy Intake/Expenditure, and Metabolism
Calcitonin gene-related peptide (CGRP) is a 37-amino acid neuropeptide expressed both centrally and peripherally. CGRP has been shown to be involved in arteriolar dilation, cardiovascular regulation, pain transmission, migraine, and gastrointestinal physiology. Our current research is aimed at analyzing CGRP’s impact on appetite/satiety, body metabolism, and energy homeostasis. Our study investigated the effects of a single-dose intraperitoneal (IP) treatment with CGRP on food and water consumption, energy expenditure, physical activity, respirometry, and a panel of plasma metabolic hormones in C57Bl/6 wild-type (WT) mice. After a CGRP IP injection at a dose of 2 nmol (10 μM CGRP in 200 μl of saline), a significant reduction in food intake and metabolic parameters as RQ, VCO2, and VO2 was observed. CGRP-injected mice had also significantly lower total energy expenditure (TEE) with no changes in activity levels compared to vehicle-injected controls. CGRP treatment in mice induced significantly lower plasma levels of glucagon and leptin but higher levels of amylin. Our data show that a single dose of CGRP peptide significantly decreased food consumption and altered calorimetric parameters and plasma metabolic hormone levels, thus confirming that CGRP plays a pivotal role in the regulation of appetite and metabolism. Future studies are necessary to analyze CGRP’s long-term impact on body metabolism and its potential effects on appetite, obesity, and metabolic disorders.
KeywordsCalcitonin gene-related peptide Metabolism Appetite Metabolic hormones
D.S., J.P.V., J.R.P., and P.G. conception and design of research; D.S., J.P.V., L.L., S.O., A.G., J.R.P., and P.G. performed experiments, D.S., J.P.V., L.L., S.O., A.G., J.R.P., and P.G. analyzed data; D.S., J.P.V., L.L., S.O., A.G., J.R.P., and P.G. interpreted results of experiments; D.S., J.P.V., J.R.P., and P.G. prepared figures; D.S., J.P.V., J.R.P., and P.G. drafted manuscript; D.S., J.P.V., L.L., S.O., A.G., J.R.P., and P.G. edited and revised manuscript; D.S., J.P.V., L.L., S.O., A.G., J.R.P., and P.G. approved final version of manuscript.
Compliance with Ethical Standards
VA Merit Review I01RX000873 (Germano, P).
VA ShEEP IS1BX003075 (Germano, P).
VA ShEEP IS1BX003553 (Germano, P).
NIH NIDDK P30 NIH DK41301.
NIH NIDDK U01 CSCPDPC -CCs -RFA-DK-14-027.
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