Co-Administration of Progesterone and Melatonin Attenuates Ischemia-Induced Hippocampal Damage in Rats
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Stroke is the second leading reason for death worldwide and is one of the fundamental causes of long-term disabilities. The aim of this investigation was to assess the impact of combined administration progesterone (PROG) and melatonin (MEL) on stroke complications. Male Wistar rats (9–10 weeks) weighing 250–300 g were used as a part of this examination. They were randomly separated into eight groups (nine rats for every group). Common carotid arteries on the two sides clamped (BCCAO model) with non-traumatic clips for 20 min. At that point, the rats were treated with 8 mg/kg PROG, 10 mg/kg MEL, and vehicles (sesamoid and normal saline). Morris water maze testing was performed following 2 weeks. At that point, the rats were euthanized, and histological examination was directed. The outcome demonstrated that utilization of PROG and MEL in treatment groups essentially increases the quantity of pyramidal cells and enhances spatial memory compared to non-treatment groups (p < 0.05). Moreover, the neuroleptic factor gene expression and protein concentration were significantly enhanced in the treated groups (p < 0.05). As indicated by the outcomes, co-administration of PROG and MEL can enhance learning and memory by surviving the pyramidal neurons and diminishing neural death by means of increasing neuroleptic factors in the hippocampal CA1 zone.
KeywordsGlobal brain ischemia Progesterone Melatonin Apoptosis and neurotrophic factors
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Conflict of Interest
The authors declare that they have no conflict of interest.
- Chen TY, Lee MY, Chen HY, Kuo YL, Lin SC, Wu TS, Lee E (2006) Melatonin attenuates the postischemic increase in blood–brain barrier permeability and decreases hemorrhagic transformation of tissue-plasminogen activator therapy following ischemic stroke in mice. J Pineal Res 40:242–250CrossRefPubMedGoogle Scholar
- Letechipía-Vallejo G, López-Loeza E, ESPINOZA-GONZÁLEZ V, González-Burgos I, Olvera-Cortés ME, Moralí G, Cervantes M (2007) Long-term morphological and functional evaluation of the neuroprotective effects of post-ischemic treatment with melatonin in rats. J Pineal Res 42:138–146CrossRefPubMedGoogle Scholar
- Sharifi Z-N, Abolhassani F, Zarrindast MR, Movassaghi S, Rahimian N, Hassanzadeh G (2011) Effects of FK506 on hippocampal CA1 cells following transient global ischemia/reperfusion in Wistar rat. Stroke Res Treat 2012Google Scholar
- Xu Q, Ji X-F, Chi T-Y, Liu P, Jin G, Gu S-L, Zou L-B (2015) Sigma 1 receptor activation regulates brain-derived neurotrophic factor through NR2A-CaMKIV-TORC1 pathway to rescue the impairment of learning and memory induced by brain ischaemia/reperfusion. Psychopharmacology 232:1779–1791CrossRefPubMedGoogle Scholar
- Younan N, El-Attar S & Sabry D (2012). Progesterone as a neuroprotective treatment in brain ischemic injury in Ovariectomized rats: relation to brain derived neurotrophic factor, Synaptic Plasticity and Astrocytes The Medical Journal of Cairo University, 80Google Scholar