MeCP2 Differentially Regulate the Myelin MBP and PLP Protein Expression in Oligodendrocytes and C6 Glioma
MeCP2 (methyl-CpG binding protein 2), an epigenetic regulator, has been shown to regulate the function of neurons and glial cells. Our previous study has demonstrated that MeCP2 repress the myelin gene expression in rat oligodendrocytes but whether MeCP2 bind to myelin gene MBP and PLP is not yet known. Besides oligodendrocytes, C6 glioma also expresses myelin genes and could be used as a model system to study myelin gene expression. In the present study, we determined that MeCP2 directly bind to MBP, PLP, and BDNF promoter in oligodendrocytes. Further, it was found that MeCP2 differentially regulates the myelin gene expression in oligodendrocytes and C6 glioma. In contrast to oligodendrocytes, MeCP2 does not bind to promoter region of MBP and PLP in C6 glioma suggest that MeCP2 differentially regulates the gene expression in different cell types.
KeywordsMeCP2 C6 glioma MBP PLP Oligodendrocytes
We are grateful to Prof. M. Cristina Cardoso, Department of Biology, TU Darmstadt, for her kind favor in appreciating this study by providing MeCP2 plasmid. We are also thankful to Dr. R.V. Devkar laboratory, Department of Zoology, The M.S. University of Baroda, for allowing us to carry out Fluorescent staining observations and photography using the Floid cell imaging system.
This study was funded by the Department of Biotechnology (DBT), New Delhi, India (Sanction order No. BT/PR4974/MED/30/773/2012 dated 10/09/2013).
Compliance with Ethical Standard
Conflict of Interest
The authors declare that they have no conflict of interest.
- Bottenstein, J. E., & Sato, G. H. (1979). Growth of a rat neuroblastoma cell line in serum-free supplemented medium. Proceedings of the National Academy of Sciences, 76(1):514–517Google Scholar
- Khorshid Ahmad T, Zhou T, AlTaweel K, Cortes C, Lillico R, Lakowski TM et al (2017) Experimental autoimmune encephalomyelitis (EAE)-induced elevated expression of the E1 isoform of methyl CpG binding protein 2 (MeCP2E1): implications in multiple sclerosis (MS)-induced neurological disability and associated myelin damage. Int J Mol Sci 18(6):1254CrossRefPubMedCentralGoogle Scholar
- Livak, K. J., & Schmittgen, T. D. (2001). Analysis of relative gene expression data using real-time quantitative PCR and the 2− ΔΔCT method. methods, 25(4):402–408Google Scholar
- Quarles RH, Macklin WB, Morell P (2006) Myelin formation, structure and biochemistry. Basic neurochemistry: molecular, cellular and medical aspects 7:51–71Google Scholar
- Yakabe S, Soejima H, Yatsuki H, Tominaga H, Zhao W, Higashimoto K, Joh K, Kudo S, Miyazaki K, Mukai T (2008) MeCP2 knockdown reveals DNA methylation-independent gene repression of target genes in living cells and a bias in the cellular location of target gene products. Genes Genet Syst 83(2):199–208CrossRefPubMedGoogle Scholar