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Journal of Molecular Neuroscience

, Volume 65, Issue 3, pp 343–350 | Cite as

MeCP2 Differentially Regulate the Myelin MBP and PLP Protein Expression in Oligodendrocytes and C6 Glioma

  • Kedarlal Sharma
  • Juhi Singh
  • Prakash P. Pillai
Article

Abstract

MeCP2 (methyl-CpG binding protein 2), an epigenetic regulator, has been shown to regulate the function of neurons and glial cells. Our previous study has demonstrated that MeCP2 repress the myelin gene expression in rat oligodendrocytes but whether MeCP2 bind to myelin gene MBP and PLP is not yet known. Besides oligodendrocytes, C6 glioma also expresses myelin genes and could be used as a model system to study myelin gene expression. In the present study, we determined that MeCP2 directly bind to MBP, PLP, and BDNF promoter in oligodendrocytes. Further, it was found that MeCP2 differentially regulates the myelin gene expression in oligodendrocytes and C6 glioma. In contrast to oligodendrocytes, MeCP2 does not bind to promoter region of MBP and PLP in C6 glioma suggest that MeCP2 differentially regulates the gene expression in different cell types.

Keywords

MeCP2 C6 glioma MBP PLP Oligodendrocytes 

Notes

Acknowledgments

We are grateful to Prof. M. Cristina Cardoso, Department of Biology, TU Darmstadt, for her kind favor in appreciating this study by providing MeCP2 plasmid. We are also thankful to Dr. R.V. Devkar laboratory, Department of Zoology, The M.S. University of Baroda, for allowing us to carry out Fluorescent staining observations and photography using the Floid cell imaging system.

Funding

This study was funded by the Department of Biotechnology (DBT), New Delhi, India (Sanction order No. BT/PR4974/MED/30/773/2012 dated 10/09/2013).

Compliance with Ethical Standard

Conflict of Interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Division of NeurobiologyThe M. S. University of BarodaVadodaraIndia
  2. 2.Department of ZoologyThe M. S. University of BarodaVadodaraIndia
  3. 3.Faculty of ScienceThe M. S. University of BarodaVadodaraIndia

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