Journal of Molecular Neuroscience

, Volume 65, Issue 2, pp 203–212 | Cite as

Hispidulin Protects Against Focal Cerebral Ischemia Reperfusion Injury in Rats

  • Pengpeng An
  • Tianhui Wu
  • Huanqing Yu
  • Kun Fang
  • Zhizhen Ren
  • Ming TangEmail author


Focal cerebral ischemia is associated with ischemia/reperfusion (I/R) injury. Hispidulin is a flavonoid compound with a variety of pharmacological properties. The neuroprotective effects of hispidulin have not been fully elucidated. Herein, we demonstrated that pretreatment of animals with hispidulin improved the neurological outcomes and decreased the infarct size and brain edema in the cerebral focal I/R model. Mechanistically, we showed in vivo and in vitro that hispidulin exerted a protective effect against I/R injury by inducing the Nrf2 antioxidant pathway through modulation of AMPK/GSK3β signaling. Taken together, our results suggest that hispidulin may be a useful neuroprotective agent against ischemia/reperfusion (I/R) injury.


Hispidulin Ischemia/reperfusion (I/R) injury Nrf2 AMPK/GSK3β 



The study was supported by Shandong Province Chinese Medicine Science and Technology Development Project (2017-321) and Qingdao medical scientific research guidance program (2016-WJZD024).

Compliance with Ethical Standards

The experimental protocol was approved by the Medical Ethics Committee of The Affiliated Qingdao Hiser Hospital of Qingdao University.

Conflict of Interest

The authors declare that they have no conflict of interest.

Supplementary material

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(GIF 36 kb)

12031_2018_1086_MOESM1_ESM.tif (817 kb)
High resolution image (TIF 817 kb)


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© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.The Affiliated Qingdao Hiser Hospital of Qingdao University (Qingdao Hospital of Traditional Chinese Medicine)QingdaoChina
  2. 2.Qingdao Fifth People’s Hospital (Shandong Qingdao Hospital of Integrated Traditional and Western Medicine)QingdaoChina
  3. 3.Community Health Service Center of Shinan DistrictQingdaoChina

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