Expression Analysis of Vitamin D Signaling Pathway Genes in Epileptic Patients
- 88 Downloads
Vitamin D deficiency has been detected in epileptic patients. Vitamin D participates in neuroprotection, brain cell proliferation, and differentiation. Consequently, vitamin D supplementation has been suggested as an alternative treatment in epileptic patients. We aimed at assessment of vitamin D signaling pathway in epileptic patients. In the present study, we evaluated vitamin D serum concentration as well as expression of vitamin D receptor (VDR) gene and genes encoding for vitamin D activating enzyme 1-alpha-hydroxylase (CYP27B1) and deactivating enzyme 24-hyroxylase (CYP24A1) in epileptic patients compared with healthy individuals. We found significant lower levels of vitamin D in epileptic patients compared with healthy subjects. Expression analyses showed significant downregulation of VDR expression in peripheral blood of epileptic patients compared with healthy subjects (relative expression (REx) = 0.16, P < 0.001). However, there was no significant difference in CYP24A1 expression between epileptic patients and normal subjects. CYP27B1 expression analysis showed significant upregulation in male patients aged between 30 and 40 (REx = 5.43, P = 0.013). After using two-way ANCOVA for adjusting the effects of sex and age, there was a statistically significant difference in the VDR expression values between patient and control groups (P < 0.001). Spearman’s correlation analysis showed no significant correlation between genes expression levels and patients’ age or vitamin D serum concentrations. However, we found significant correlations between VDR expression levels and CYP24A1/ CYP27B1 expression levels in epileptic patients (r = 0.435 and P < 0.001; r = 0.26 and P = 0.02 respectively). There was also a significant correlation between the expression levels of CYP24A1 and CYP27B1 (r = 0.349 and P = 0.001). Our study shows a possible role for VDR in the pathogenesis of epilepsy.
KeywordsVitamin D VDR CYP27B1 CYP24A1 Epilepsy
The study was funded by Vice-chancellor for Research and Technology, Hamadan University of Medical Sciences (No. 9412187285).
- Clinckspoor I, Hauben E, Verlinden L, Van Den Bruel A, Vanwalleghem L, Poorten VV, Delaere P, Mathieu C, Verstuyf A, Decallonne B (2012) Altered expression of key players in vitamin d metabolism and signaling in malignant and benign thyroid tumors. J Histochem Cytochem 60:502–511CrossRefPubMedPubMedCentralGoogle Scholar
- Janik S, Nowak U, Laszkiewicz A, Satyr A, Majkowski M, Marchwicka A, Sniezewski L, Berkowska K, Gabrys M, Cebrat M and Marcinkowska E (2017) Diverse regulation of vitamin D receptor gene expression by 1,25-Dihydroxyvitamin D and ATRA in murine and human blood cells at early stages of their differentiation. Int J Mol Sci 18. https://doi.org/10.3390/ijms18061323