Journal of Molecular Neuroscience

, Volume 64, Issue 2, pp 242–251 | Cite as

Evaluation of the Mitochondria-Related Redox and Bioenergetics Effects of Gastrodin in SH-SY5Y Cells Exposed to Hydrogen Peroxide

  • Marcos Roberto de OliveiraEmail author
  • Flávia Bittencourt Brasil
  • Cristina Ribas Fürstenau


Mitochondrion is the main site of ATP production in animal cells and also orchestrates signaling pathways associated with cell survival and death. Mitochondrial dysfunction has been linked to bioenergetics and redox impairment in human diseases, such as neurodegeneration and cardiovascular disease. Protective agents able to attenuate mitochondrial impairment are of pharmacological interest. Gastrodin (GAS; 4-hydroxybenzyl alcohol 4-O-beta-d-glucoside) is a phenolic glucoside obtained from the Chinese herbal medicine Gastrodia elata Blume and exhibits antioxidant, anti-inflammatory, and antiapoptotic effects in several cell types. GAS is able to cross the blood-brain barrier, reducing the impact of different stressors on the cognition of experimental animals. In the present work, we investigated whether GAS would protect mitochondria of human SH-SY5Y neuroblastoma cells against an exposure to a pro-oxidant agent. The cells were treated with GAS at 25 μM for 30 min before the administration of hydrogen peroxide (H2O2) at 300 μM for an additional 3 or 24 h, depending on the assay. We evaluated both mitochondrial redox state and function parameters and analyzed the mechanism by which GAS protected mitochondria in this experimental model. Silencing of the nuclear factor erythroid 2-related factor 2 (Nrf2) transcription factor suppressed the GAS-induced mitochondrial protection seen here. Moreover, Nrf2 knockdown abrogated the effects of GAS on cell viability, indicating a potential role for Nrf2 in both mitochondrial and cellular protection promoted by GAS. Further research would be necessary to investigate whether GAS would be able to induce similar effects in in vivo experimental models.


Gastrodin Mitochondria Bioenergetics Redox impairment Nrf2 



This work was supported by CNPq. FBB receives financial support from the FOPESQ/UFF.

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Marcos Roberto de Oliveira
    • 1
    Email author
  • Flávia Bittencourt Brasil
    • 2
  • Cristina Ribas Fürstenau
    • 3
  1. 1.Department of Chemistry/ICETFederal University of Mato Grosso (UFMT)CuiabaBrazil
  2. 2.Universidade Federal FluminenseRio de JaneiroBrazil
  3. 3.Instituto de Genética e Bioquímica (INGEB)Universidade Federal de Uberlândia (UFU)Patos de MinasBrazil

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