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Journal of Molecular Neuroscience

, Volume 64, Issue 2, pp 262–272 | Cite as

YAP Promotes Migration and Invasion of Human Glioma Cells

  • Yu Zhang
  • Peng Xie
  • Xu Wang
  • Peng Pan
  • Yan Wang
  • Hao Zhang
  • Yu Dong
  • Yi Shi
  • Yang Jiang
  • Rutong YuEmail author
  • Xiuping ZhouEmail author
Article

Abstract

Previously, we have reported that Yes-associated protein (YAP) is upregulated in human glioma tissues and its level is positively correlated with patient prognosis. However, the role and mechanism of YAP in the highly invasive nature of human gliomas were largely unknown. In this study, examined by wound healing assay, transwell assay, or live-imaging, we found that YAP downregulation inhibited glioma cell migration and invasion, while YAP over-expression promoted them. Interestingly, the above effect of YAP on immortalized glioma cells was recapitulated in cultured primary glioma cells. In addition, the protein level of N-cadherin and Twist, two important proteins involved in tumor invasion, increased after YAP over-expression. Meanwhile, YAP over-expression significantly increased the F-actin level and changed the distribution of F-actin, leading to cytoskeletal reorganization, which plays an important role in cell motility. Furthermore, the promotion effect of YAP over-expression on glioma cell migration and invasion was partially abolished by Twist downregulation. Taken together, our findings show that YAP contributes to glioma cell migration and invasion by regulating N-cadherin and Twist, as well as cytoskeletal reorganization.

Keywords

Glioma YAP Migration F-actin N-cadherin Twist 

Notes

Acknowledgements

We thank Professor Chunmei Zhu in the School of International Education of Xuzhou Medical University for the English writing assistance.

Funding Information

This study was supported by National Natural Science Foundation of China (No. 81372699; No. 81672489; No.81472345) and the Six Major Talent Summit of Jiangsu Province (No. WSW-039).

Compliance with Ethical Standards

Written informed consent was obtained from the patients and the study was approved by the ethics committee of the hospital.

Supplementary material

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.The Graduate SchoolXuzhou Medical UniversityXuzhouChina
  2. 2.Department of NeurosurgeryHuaian Second People’s HospitalHuaianChina
  3. 3.Brain HospitalAffiliated Hospital of Xuzhou Medical UniversityXuzhouPeople’s Republic of China
  4. 4.Institute of Nervous System DiseasesXuzhou Medical UniversityXuzhouPeople’s Republic of China

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