Oxygen Supplementation Improves Protein Milieu Supportive of Protein Synthesis and Antioxidant Function in the Cortex of Alzheimer’s Disease Model Mice—a Quantitative Proteomic Study
- 152 Downloads
Protein synthesis has been reported to be impaired in early-stage Alzheimer’s disease (AD). Previously, we found that oxygen supplementation improved cognitive function and reduced mitochondrial damage in AD model mice. In the present study, we examined the effects of supplemental oxygen treatment on protein synthesis and oxidative damage. The synthesis of numerous proteins involved in mRNA splicing, transcription regulation, and translation was found to be significantly upregulated in cortex tissues of AD model mice given a supplemental oxygen treatment (OT group), relative to those of non-treated control AD model mice (Ctrl group), suggesting that impairment in protein synthesis may be alleviated by increased oxygen inhalation. Methionine oxidation and oxidation levels in general were similar between the OT and Ctrl groups, indicating that the oxygen supplementation treatment did not cause increases in peptide oxidation levels. On the contrary, the OT group exhibited upregulation of several proteins associated with antioxidant defense. These results support further exploration into the development of supplementary oxygen treatment as a potential therapy for AD.
KeywordsAlzheimer’s disease Protein synthesis Oxygen Oxidative stress Antioxidant defense Quantitative proteomics
This work was supported by the Key Programs of Shenzhen (NO. JSGG20140703163838793) and the Basic Research Project of Shenzhen (NO. 20150006).
Compliance with Ethical Standards
Conflict of Interest
The authors declare that they have no conflict of interest.
- Bai BH, Hales CM, Chen PC, Gozal Y, Dammer EB, Fritz JJ, Wang X, Xia Q, Duong DM, Street C, Cantero G, Cheng D, Jones DR, Wu Z, Li Y, Diner I, Heilman CJ, Rees HD, Wu H, Lin L, Szulwach KE, Gearing M, Mufson EJ, Bennett DA, Montine TJ, Seyfried NT, Wingo TS, Sun YE, Jin P, Hanfelt J, Willcock DM, Levey A, Lah JJ, Peng J (2013) U1 small nuclear ribonucleoprotein complex and RNA splicing alterations in Alzheimer’s disease. Proc Natl Acad Sci 110:16562–16567CrossRefPubMedPubMedCentralGoogle Scholar
- Neeta Thakur PDB, Julian LJ, Yelin EH, Katz PP, Sidney S, Iribarren C, Eisner MD (2010) COPD and cognitive impairment: the role of hypoxemia and oxygen therapy. Int J Chron Obstruct Pulmon Dis 5:7Google Scholar
- Shim, J.S., Song, M.-Y., Yim, S.-V., Lee, S.-E., and Park, K.-S. (2016) Global analysis of ginsenoside Rg1 protective effects in β-amyloid-treated neuronal cells. J Ginseng Res. https://doi.org/10.1016/j.jgr.2016.12.003