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Journal of Molecular Neuroscience

, Volume 62, Issue 2, pp 163–180 | Cite as

Crossreactivity of an Antiserum Directed to the Gram-Negative Bacterium Neisseria gonorrhoeae with the SNARE-Complex Protein Snap23 Correlates to Impaired Exocytosis in SH-SY5Y Cells

  • A. Almamy
  • C. Schwerk
  • H. Schroten
  • H. Ishikawa
  • A. R. Asif
  • B. Reuss
Article

Abstract

Early maternal infections with Neisseria gonorrhoeae (NG) correlate to an increased lifetime schizophrenia risk for the offspring, which might be due to an immune-mediated mechanism. Here, we investigated the interactions of polyclonal antisera to NG (α-NG) with a first trimester prenatal brain multiprotein array, revealing among others the SNARE-complex protein Snap23 as a target antigen for α-NG. This interaction was confirmed by Western blot analysis with a recombinant Snap23 protein, whereas the closely related Snap25 failed to interact with α-NG. Furthermore, a polyclonal antiserum to the closely related bacterium Neisseria meningitidis (α-NM) failed to interact with both proteins. Functionally, in SH-SY5Y cells, α-NG pretreatment interfered with both insulin-induced vesicle recycling, as revealed by uptake of the fluorescent endocytosis marker FM1-43, and insulin-dependent membrane translocation of the glucose transporter GluT4. Similar effects could be observed for an antiserum raised directly to Snap23, whereas a serum to Snap25 failed to do so. In conclusion, Snap23 seems to be a possible immune target for anti-gonococcal antibodies, the interactions of which seem at least in vitro to interfere with vesicle-associated exocytosis. Whether these changes contribute to the correlation between maternal gonococcal infections and psychosis in vivo remains still to be clarified.

Keywords

Neisseria gonorrhoeae Neisseria meningitidis Snap23 Exocytosis GluT4 SH-SY5Y cells 

Notes

Acknowledgments

We would like to thank the University Medicine Göttingen (UMG) for persistent and reliable support of our work.

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no competing interests.

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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  • A. Almamy
    • 1
  • C. Schwerk
    • 2
  • H. Schroten
    • 2
  • H. Ishikawa
    • 3
  • A. R. Asif
    • 4
  • B. Reuss
    • 1
  1. 1.Institute for NeuroanatomyUniversity Medical Center GöttingenGöttingenFederal Republic of Germany
  2. 2.University Children’s Hospital Mannheim, Pediatric Infectious DiseasesHeidelberg UniversityHeidelbergGermany
  3. 3.The Nippon Dental UniversityTokyoJapan
  4. 4.Institute for Clinical ChemistryUniversity Medical Center GöttingenGöttingenGermany

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