Journal of Molecular Neuroscience

, Volume 60, Issue 2, pp 157–170

Capsaicin-Sensitive Sensory Nerves Mediate the Cellular and Microvascular Effects of H2S via TRPA1 Receptor Activation and Neuropeptide Release

  • Zsófia Hajna
  • Éva Sághy
  • Maja Payrits
  • Aisah A. Aubdool
  • Éva Szőke
  • Gábor Pozsgai
  • István Z. Bátai
  • Lívia Nagy
  • Dániel Filotás
  • Zsuzsanna Helyes
  • Susan D. Brain
  • Erika Pintér
Article

Abstract

It is supposed that TRPA1 receptor can be activated by hydrogen sulphide (H2S). Here, we have investigated the role of TRPA1 receptor in H2S-induced [Ca2+]i increase in trigeminal ganglia (TRG) neurons, and the involvement of capsaicin-sensitive sensory nerves in H2S-evoked cutaneous vasodilatation. [Ca2+]i was measured with ratiometric technique on TRG neurons of TRPA1+/+ and TRPA1−/− mice after NaHS, Na2S, allylisothiocyanate (AITC) or KCl treatment. Microcirculatory changes in the ear were detected by laser Doppler imaging in response to topical NaHS, AITC, NaOH, NaSO3 or NaCl. Mice were either treated with resiniferatoxin (RTX), or CGRP antagonist BIBN4096, or NK1 receptor antagonist CP99994, or K+ATP channel blocker glibenclamide. Alpha-CGRP−/− and NK1−/− mice were also investigated. NaHS and Na2S increased [Ca2+]i in TRG neurons derived from TRPA+/+ but not from TRPA1−/− mice. NaHS increased cutaneous blood flow, while NaOH, NaSO3 and NaCl did not cause significant changes. NaHS-induced vasodilatation was reduced in RTX-treated animals, as well as by pre-treatment with BIBN4096 or CP99994 alone or in combination. NaHS-induced vasodilatation was significantly smaller in alpha-CGRP−/− or NK1−/− mice compared to wild-types. H2S activates capsaicin-sensitive sensory nerves through TRPA1 receptors and the resultant vasodilatation is mediated by the release of vasoactive sensory neuropeptides CGRP and substance P.

Keywords

Capsaicin-sensitive sensory neuron CGRP Hydrogen sulphide Substance P TRPA1 receptor 

Abbreviations

AITC

allylisothiocyanate

CGRP

calcitonin gene-related peptide

CHO

Chinese hamster ovary

CPM

counts per minute

DRG

dorsal root ganglion

H2S

hydrogen sulphide

NK1

neurokinin 1

NK2

neurokinin 2

NO

nitric oxide

HNO

nitroxyl

RTX

resiniferatoxin

SP

substance P

TRPA1

Transient Receptor Potential Ankyrin 1

TRPV1

Transient Receptor Potential Vanilloid 1

TRG

trigeminal ganglion

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Zsófia Hajna
    • 1
    • 2
  • Éva Sághy
    • 1
    • 2
  • Maja Payrits
    • 1
    • 2
  • Aisah A. Aubdool
    • 4
  • Éva Szőke
    • 1
    • 2
    • 3
  • Gábor Pozsgai
    • 1
    • 2
  • István Z. Bátai
    • 1
    • 2
  • Lívia Nagy
    • 2
  • Dániel Filotás
    • 5
  • Zsuzsanna Helyes
    • 1
    • 2
    • 3
  • Susan D. Brain
    • 4
  • Erika Pintér
    • 1
    • 2
  1. 1.Department of Pharmacology and Pharmacotherapy, Faculty of MedicineUniversity of PécsPécsHungary
  2. 2.János Szentágothai Research CentreUniversity of PécsPécsHungary
  3. 3.MTA-PTE NAP B Chronic Pain Research GroupPécsHungary
  4. 4.BHF Cardiovascular Centre of Excellence and Centre of Integrative BiomedicineKing’s College LondonLondonUK
  5. 5.Department of General and Physical Chemistry, Faculty of SciencesUniversity of PécsPécsHungary

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