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Journal of Molecular Neuroscience

, Volume 56, Issue 4, pp 949–955 | Cite as

Pygo2 siRNA Inhibit the Growth and Increase Apoptosis of U251 Cell by Suppressing Histone H3K4 Trimethylation

  • Mingcong Li
  • Linlin Chao
  • Jian Wu
  • Hao Xu
  • Shanghan Shen
  • Sifang Chen
  • Xin Gao
  • Ning Yu
  • Zhanxiang WangEmail author
Article

Abstract

The development of novel therapeutic strategies for glioma requires the identification of molecular targets involved in malignancy. Pygopus (Pygo) is a new discovered and specific downstream component of canonical Wnt signaling. Our previous study has demonstrated that Pygo2 is highly expressed in and promotes the growth of glioma cells. However, the role of Pygo2 in glioma remains to be elucidated. In the current study, we investigated the role of Pygo2 in human glioma U251 cells and showed that knocking down of the expression of Pygo2 in U251 cells using lentivirally expressed siRNA have inhibited cell proliferation and increased apoptosis through decreasing H3K4me3 expression. Moreover, we found Pygo2 was enriched in U251 glioma cancer stem-like cells and Pygo2 siRNA resulted in a reduced number as well as size of tumor spheres. According to our result, this paper now links mechanistically Pygo2’s role in histone modification to its enhancement/reduction of proliferation/apoptosis in glioma cells and indicate that Pygo2 may play an important role in self-renew and proliferation in U251 glioma cancer stem-like cells.

Keywords

Pygo2 Growth Apoptosis H3K4me3 

Abbreviations

CNS

Central nervous system

LEF/TCF

Lymphoid-enhancing factor 1/T-cell factor

H3K4me3

Trimethylation of histone H3 at lysine 4

EGF

Epidermal growth factor

bFGF

Basic fibroblast growth factor

GFP

Green fluorescent protein

GAPDH

Glyceraldehyde-3-phosphate dehydrogenase

TUNEL

Terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling

DAPI

4′,6-diamidino-2-phenylindole

Notes

Acknowledgments

This work was supported by the National Natural Science Foundation of China (no. 81271332) and Fujiani Natural Science Foundation (2009D002).

Conflict of Interest

All the authors of this study declare no conflict of interest.

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Mingcong Li
    • 1
  • Linlin Chao
    • 1
  • Jian Wu
    • 3
  • Hao Xu
    • 2
  • Shanghan Shen
    • 3
  • Sifang Chen
    • 3
  • Xin Gao
    • 1
  • Ning Yu
    • 3
  • Zhanxiang Wang
    • 3
    Email author
  1. 1.The First Clinical Medical CollegeFujian Medical UniversityFuzhouThe People’s Republic of China
  2. 2.Department of NeurosurgeryAnhui Provincial HospitalHefeiPeople’s Republic of China
  3. 3.Department of NeurosurgeryThe First Affiliated Hospital of Xiamen UniversityXiamenThe People’s Republic of China

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